弥勒市葡萄灰霉病菌对4种杀菌剂的抗药性检测  

Evaluation on resistance of Botrytis cinerea to four fungicides in Mile county

作　　者：普继雄 周宗山 王娜 冀志蕊 张俊祥[3] PU Jixiong;ZHOU Zongshan;WANG Na;JI Zhirui;ZHANG Junxiang(Mile County Agricultural Technology Extension Center,Mile 652399,Yunnan,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Germplasm Resources Utilization),Ministry of Agriculture and Rural Affairs,Xingcheng 125100,Liaoning,China;Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,Liaoning,China)

机构地区：[1]弥勒市农业技术推广中心,云南弥勒652399 [2]农业农村部园艺作物种质资源利用重点实验室,辽宁兴城125100 [3]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《果树学报》2021年第7期1147-1152,共6页Journal of Fruit Science

基　　金：国家重点研发计划(2018YFD0201301);中国农业科学院科技创新工程(CAAS-ASTIP)。

摘　　要：【目的】灰霉病是葡萄生产过程中的一种重要病害,严重影响葡萄的产量和商品价值。明确弥勒市不同葡萄产区灰霉病菌对腐霉利、嘧霉胺、咯菌腈和氟吡菌酰胺的抗药状况,为葡萄灰霉病的化学防治提供理论依据。【方法】从弥勒市市辖的12个乡、镇(农场)采集葡萄灰霉病样本,分离和纯化得到197株灰霉病菌菌株。采用菌丝生长速率法测定197株灰霉病菌对4种农药的敏感性。【结果】葡萄灰霉病菌对腐霉利、嘧霉胺抗药频率分别为71.1%、100.0%,高抗频率分别为13.7%和23.8%;除在弥勒市弥阳地区检测到2株低抗咯菌腈菌株,其他地区未检测到抗咯菌腈菌株;分离的葡萄灰霉病菌均对氟吡菌酰胺敏感。【结论】弥勒地区葡萄灰霉病菌对腐霉利和嘧霉胺产生了严重的抗药性,腐霉利和嘧霉胺双重抗性频率为71.1%。在实际生产中,建议以咯菌腈或氟吡菌酰胺与生物农药交替使用进行葡萄灰霉病的防治。【Objective】Grape grey mould,caused by Botrytis cinerea,brings about damage to grapes(Vitis vinifera)in vineyards throughout the world.The first signs of disease on grapes often appear during the bloom period,when the fungus attacks the flower.The infected berries may become covered with greyish-tan conidia of the fungus.Berry stalks and cluster stems may be invaded,causing them to shrivel,and berries that have split or have been punctured are often easily attacked by other organisms.Gray mold is one of the most serious fungal diseases in grape.Due to the lack of registered broad-spectrum compounds,effective control of grape grey mould is usually based on repeated fungicide applications during the growing season,especially in mild and humid climates.But the fungus is known to rapidly adapt to its environment and to develop fungicide resistance in the field.Because of these problems,the pesticide resistance management strategies have now been widely adopted,which restrict the usage of the same fungicide.However,increasing occurrence of isolates with low-level cross-resistance to various fungicides has been observed in Chinese vineyards.In Mile grape production,the following fungicide groups are in current use against B.cinerea:procymidone,pyrimethanil,fludioxonil and fluopyram.However,little is known about the pesticide resistance in B.cinerea populations from the grape in Mile county,which is the primary region for grape production in Yunnan province.The objectives of this study were to:(1)assess the resistance to procymidone and pyrimethanil and establish the resistance distribution of B.cinerea to procymidone and pyrimethanil in Mile county;(2)assess the stability of resistance to fludioxonil;(3)estimate the stability of resistance to fluopyram;(4)evaluate the efficacy of the four fungicides for controlling grape gray mold.【Methods】The diseased samples were collected from Mile county in Yunan province.One hundred and ninety-seven strains of B.cinerea were isolated and purified.All isolates were then sing

关 键 词：葡萄 灰葡萄孢菌 腐霉利 嘧霉胺 咯菌腈 氟吡菌酰胺 

分 类 号：S663.1[农业科学—果树学]