不同浓度艾烟环境对大鼠鼻黏膜损伤及血清IL-1、IL-6和TNF-α表达的影响  

Effect of different concentrations of moxa smoke exposure on nasal mucosal injury and expression of serum IL-1, IL-6 and TNF-α in rats

作　　者：蒋志明 魏小桐 赵丽娜 吴立斌 刘磊[2] 李小贾 王敏君 胡玲[2,3] 吴子建 JIANG Zhi-ming;WEI Xiao-tong;ZHAO Li-na;WU Li-bin;LIU Lei;LI Xiao-jia;WANG Min-jun;HU Ling;WU Zi-jian(Graduate School,Anhui University of CM,Hefei 230012,China;College of Acupuncture-Moxibustion and Tuina,Anhui University of CM,Hefei 230012,China;Institute of Acupuncture-Moxibustion and Meridians,Anhui Academy of TCM,Hefei 230038)

机构地区：[1]安徽中医药大学研究生院,合肥230012 [2]安徽中医药大学针灸推拿学院,合肥230012 [3]安徽省中医药科学院针灸经络研究所,合肥230038

出　　处：《中国针灸》2021年第7期781-786,共6页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(“973”计划)项目:2015CB554504;安徽中医药大学探索科研项目:2016ts024。

摘　　要：目的:观察长时间不同浓度艾烟环境对大鼠嗅觉功能的影响,为艾灸过程产生的艾烟安全性研究提供实验依据。方法:将40只SD大鼠随机分为正常对照组、低浓度艾烟组(低烟组)、中浓度艾烟组(中烟组)和高浓度艾烟组(高烟组),每组10只。艾烟组大鼠分别放入3个不同艾烟浓度有机玻璃艾灸箱内进行每日2次、每次4 h的艾烟烟熏干预,持续90 d。实验前及实验中对大鼠一般状态进行评估;干预结束后,通过双瓶实验(TBE)对大鼠进行嗅觉评估,采用HE染色观察大鼠鼻黏膜组织形态、TUNEL法检测大鼠鼻黏膜嗅上皮细胞凋亡情况,采用ELISA法检测大鼠血清白介素1(IL-1)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)的含量。结果:艾烟烟熏后期(干预45~90 d),中烟组、高烟组大鼠行为活动较正常对照组大鼠减弱,且对刺激反应强烈,精神状态较差;干预结束后,中烟组、高烟组大鼠醋水混合液饮用率均高于正常对照组、低烟组(P<0.01);中烟组、高烟组大鼠鼻黏膜层次结构不清、排列紊乱,有坏死,炎性细胞浸润严重;中烟组、高烟组大鼠鼻黏膜嗅上皮凋亡细胞数多于正常对照组、低烟组(P<0.01),高烟组多于中烟组(P<0.01);低烟组、中烟组、高烟组大鼠血清IL-1、IL-6、TNF-α含量高于正常对照组(P<0.01),且中烟组、高烟组高于低烟组(P<0.01),高烟组高于中烟组(P<0.01)。结论:长期暴露在低、中、高浓度艾烟环境中均会引起大鼠鼻黏膜组织病理改变,导致血清IL-1、IL-6、TNF-α含量升高,并且中、高浓度艾烟环境会对嗅觉功能产生一系列损害,降低大鼠嗅觉灵敏度。Objective To observe the effect of long-term moxa smoke exposure of different concentrations on olfactory function in rats, and provide experimental basis of safety study of moxa smoke produced by moxibustion. Methods Forty SD rats were randomly divided into a normal control group, a low-concentration moxa smoke group, a moderate-concentration moxa smoke group and a high-concentration moxa smoke group, 10 rats in each one. The rats in the moxa smoke groups were put into three plexiglass moxibustion boxes with different moxa smoke concentrations, 4 hours per times, twice a day for 90 days. The general state of rats was evaluated before and during the experiment. After the intervention, the olfactory function was evaluated by two-bottle experiment(TBE);the morphology of nasal mucosa was observed by HE staining;the apoptosis of olfactory epithelial cells in nasal mucosa was detected by TUNEL method;the serum levels of interleukin-1(IL-1), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were detected by ELISA method. Results In the late stage of moxa smoke exposure(45-90 days into intervention), the behavioral activity of rats in the moderate-concentration moxa smoke group and the high-concentration moxa smoke group was weaker than that in the normal control group, and their response to stimulation was strong, and their mental state was worse. After intervention, the drinking rate of vinegar-water mixture in the moderate-concentration moxa smoke group and the high-concentration moxa smoke group was higher than that in the normal control group and the low-concentration moxa smoke group(P<0.01). The hierarchical structure of nasal mucosa in the moderate-concentration moxa smoke group and the high-concentration moxa smoke group was unclear, disordered, necrotic and inflammatory cell infiltration was serious;the number of apoptotic cells in olfactory epithelium of nasal mucosa in the moderate-concentration moxa smoke group and the high-concentration moxa smoke group was more than that in the normal control group

关 键 词：艾烟吸入 嗅觉功能 嗅觉通路 炎性损伤 安全性 

分 类 号：R245.8[医药卫生—针灸推拿学]