Integrating the physical and genetic map of bread wheat facilitates the detection of chromosomal rearrangements  

作　　者：ZHAO Lai-bin XIE Die HUANG Lei ZHANG Shu-jie LUO Jiang-tao JIANG Bo NING Shun-zong ZHANG Lian-quan YUAN Zhong-wei WANG Ji-rui ZHENG You-liang LIU Deng-cai HAO Ming 

机构地区：[1]Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,P.R.China [2]Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,P.R.China [3]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第9期2333-2342,共10页农业科学学报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(2016YFD0102000)。

摘　　要：The bread wheat genome harbors a high content of repetitive DNA,which is amenable to detection and characterization using fluorescence in situ hybridization(FISH)karyotyping.An integrated genetic map was derived from a recombinant inbred population bred from a cross between a synthetic hexaploid wheat and a commercial Chinese bread wheat cultivar,based on 28 variable FISH sites and>150000 single nucleotide polymorphism(SNP)loci.The majority(20/28)of the variable FISH sites were physically located within a chromosomal region consistent with the genetic location inferred from that of their co-segregating SNP loci.The eight exceptions reflected the presence of either a translocation(1 R/1 B,1 A/7 A)or a presumptive intra-chromosomal inversion(4 A).For eight out of the nine FISH sites detected on the Chinese Spring(CS)karyotype,there was a good match with the reference genome sequence,indicating that the most recent assembly has dealt well with the problem of placing tandem repeats.The integrated genetic map produced for wheat is informative as to the location of blocks of tandemly repeated DNA and can aid in improving the quality of the genome sequence assembly in regions surrounding these blocks.

关 键 词：TRITICEAE Triticum turgidum repetitive DNA sequences fluorescence in situ hybridization(FISH) wheat genome synthetic hexaploid wheat 

分 类 号：S512.1[农业科学—作物学]