microRNA-129-5p在脓毒症患者血清中的表达及对肺内皮细胞的影响  

Expression of MicroRNA-129-5p in Serum of Sepsis Patients and Its Effect on Pulmonary Endothelial Cells

作　　者：袁江汉 袁媛[1] 周发春[1] YUAN Jianghan;YUAN yuan;ZHOU Fachun(Department of Intensive Care Medicine,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院重症医学科,重庆400016

出　　处：《中国细胞生物学学报》2021年第6期1265-1272,共8页Chinese Journal of Cell Biology

基　　金：重庆市自然科学基金面上项目(批准号:cstc2020jcyj-msxm X1096)资助的课题。

摘　　要：该研究旨在探讨miR-129-5p(microRNA-129-5p)在脓毒症患者血清中的表达及对肺内皮细胞的影响。收集临床脓毒症患者的血液样本,通过荧光实时定量PCR(RT-qPCR)检测miR-129-5p的水平并分析其表达水平与患者临床特征的关系。在肺微血管内皮细胞(HPMEC)中过表达miR-129-5p后,用Transwell和ELISA实验检测其对细胞迁移以及炎症因子释放的影响。RT-qPCR结果显示,与对照组相比,脓毒症组miR-129-5p的表达水平降低(P<0.05);与脓毒症组相比,脓毒性休克组miR-129-5p的表达水平更低(P<0.05)。miR-129-5p的表达与氧合指数呈正相关,与降钙素原、IL-1β、IL-6、TNF-α水平呈负相关(P<0.001),且脓毒性休克组的28天死亡率较脓毒症组高(P<0.05)。过表达miR-129-5p可以明显促进细胞的迁移,减少炎症因子IL-1β、IL-6、TNF-α的释放。综上所述,miR-129-5p在脓毒症模型中低表达,病情越重其水平越低,且与氧合指数、降钙素原水平相关。过表达miR-129-5p可以明显促进细胞的迁移并减少炎症因子的释放。This study was aimed to investigate the expression of miR-129-5p (microRNA-129-5p) in serum of sepsis patients and its effect on pulmonary endothelial cells.Blood samples from patients with sepsis were collected,RT-qPCR (real-time quantitative PCR) was used to detect the level of miR-129-5p and the relationship between its expression level and clinical characteristics of patients was analyzed.miR-129-5p was overexpressed in HPMEC (pulmonary microvascular endothelial cell),the effects of miR-129-5p on cell migration and the release of inflammatory cytokines were detected by Transwell and ELISA assay.RT-qPCR results showed that the expression level of miR-129-5p was decreased in the sepsis group compared with the control group (P<0.001),and the expression level of miR-129-5p in the septic shock group was lower than that in the sepsis group (P<0.05).The expression of miR-129-5p was positively correlated with oxygenation index and negatively correlated with PCT,IL-1β,IL-6 and TNF-α levels (P<0.001),and the 28-day mortality in the septic shock group was higher than that in the sepsis group (P<0.05).Overexpression of miR-129-5p could significantly promote cell migration and reduce the release of inflammatory cytokines IL-1β,IL-6 and TNF-α.In conclusion,miR-129-5p was low expressed in the sepsis model.The more severe the disease was,the lower the level of miR-129-5p was.It was correlated with oxygenation index and PCT.Overexpression of miR-129-5p can significantly promote cell migration and reduce the release of inflammatory cytokines.

关 键 词：microRNA-129-5p 脓毒症 脓毒性休克 炎症 迁移 

分 类 号：R459.7[医药卫生—急诊医学]