L-苯丙氨酰甘氨酸炔丙酯二肽的合成及性能研究  

Synthesis and Properties of L-Phenylalanyl Glycine Propargyl Ester Dipeptide

作　　者：李艳艳 宋文杰 蒋媛 危俊吾 钱杨杨 王雨佳 毕韵梅[1] Li Yanyan;Song Wenjie;Jiang Yuan;Wei Junwu;Qian Yangyang;Wang Yujia;Bi Yunmei(Yunnan Normal University,College of Chemistry and Chemical Engineering,Kunming,Yunnan 650500,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学世界》2021年第6期344-348,共5页Chemical World

基　　金：国家自然科学基金(No.21564017)资助项目。

摘　　要：采用液相合成法,高产率地合成了L-苯丙氨酰甘氨酸炔丙酯二肽,用核磁共振氢谱(^(1)H NMR)、核磁共振碳谱(^(13)C NMR)和电喷雾质谱(ESI-MS)对L-苯丙氨酰甘氨酸炔丙酯二肽进行了表征。通过加入α-糜蛋白酶、失活的α-糜蛋白酶以及胰蛋白酶,研究了L-苯丙氨酰甘氨酸炔丙酯二肽的酶解性能,结果表明,L-苯丙氨酰甘氨酸炔丙酯二肽中的肽键只能被α-糜蛋白酶酶解,具有特异性。A newL-phenylalanyl glycine propargyl ester dipeptide was synthesized with a high yield by liquid phase synthesis.The structure of product was verified by ^(1)H NMR,^(13)C NMR and ESI-MS.The enzymatic hydrolysis property of L-phenylalanyl glycine propargyl ester was studied by addingα-chymotrypsin,inactivatedα-chymotrypsin and trypsin.The results showed that the peptide bond in the dipeptide can only be cleaved efficiently byα-chymotrypsin,indicative of specificity.

关 键 词：L-苯丙氨酰甘氨酸炔丙酯 液相法 Α-糜蛋白酶 特异性 

分 类 号：O621.2[理学—有机化学]