Two new glycoalkaloids from Solanum lyratum Thunb.  

白英中两个新糖苷生物碱

作　　者：Tong Wu Xiao Du Jiannong Wang Liangyu Liu Yuke Yang 吴桐;杜肖;王建农;刘良裕;杨宇珂(中国中医科学院西苑医院基础医学研究所,北京100091)

机构地区：[1]Institute of Basic Medical Sciences ofXiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing,100091,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2021年第6期518-523,共6页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No. 81872989);the Program of G20 Project Support Guarantee(Grant No. Z151100003815026);the National Major New Drugs Inn ovation Projects (Grant No. 2009ZX09103-402)。

摘　　要：To study the alkaloids of Solanum lyratum Thunb.ODS and silica gel column chromatography combined with HPLC were used to separate and purify the alkaloids.Seven alkaloids were identified via UHPLC-ESI-Q-TOF-MS,1 D and 2 D NMR,respectively 15β-ethoxy-(3β,5α,25 R)-16,23-epoxy-23,24-imino-cholestan-16,20,23(N)-triene-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyran-oside(1),15β-hydroxyl-(3β,25 R)-16,23-epoxy-23,24-imino-cholestan-5,16,20,23(N)-tetraene-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactop-yranoside(2),Solalyraine A(3),Solalyraine B(4),16,23-epoxy-22,26-imino-cholest-22(N),23,25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(5),N-(4-amino-butyl)-3-(3-hydroxy-4-methoxy-phenyl)-E-acrylamide(6)and magnoflorine(7).Among them,compounds 1 and 2 were two new steroidal glycoalkaloids that has not been reported in the literature,and compound 7 was isolated from S.lyratum for the first time.对中药白英的生物碱类成分进行研究。采用ODS、硅胶柱层析和高效液相色谱相结合的方法对生物碱进行分离纯化。通过UHPLC-ESI-Q-TOF-MS、1D和2DNMR鉴定了7个生物碱类化合物,分别为15β-ethoxy-(3β,5α,25R)-16,23-epoxy-23,24-imino-cholestan-16,20,23(N)-triene-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(1),15β-hydroxyl-(3β,25R)-16,23-epoxy-23,24-imino-cholestan-5,16,20,23(N)-tetraene-3β-ol-3-O-β-Dglucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactop-yranoside(2),Solalyraine A(3),Solalyraine B(4),16,23-epoxy-22,26-imino-cholest-22(N),23,25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(5),N-(4-amino-butyl)-3-(3-hydroxy-4-methoxy-phenyl)-E-acrylamide(6)and magnoflorine(7)。其中化合物1和2为文献未报道的两个新的甾体糖苷类生物碱,化合物7首次从白英中分离得到。

关 键 词：Solanum lyratum Thunb. Steroidal glycoalkaloids Chemical constituents Structure identification 

分 类 号：R284.1[医药卫生—中药学]