TNF-α基因多态性与HIV感染患者抗病毒治疗后CD^(+)_(4)T细胞水平的关联性  

Association between TNF-α gene polymorphisms and CD^(+)_(4) T cell levels after antiviral therapy in HIV-infected patients

作　　者：陆宇红[1] 杨崎恩[1] 孙皆齐[1] 韩军 陈仁芳[1] LU Yu-hong;YANG Qi-en;SUN Jie-qi;HAN Jun;CHEN Ren-fang(Wuxi Infectious Disease Hos pital,Wuai,Jiangsu 214000,China)

机构地区：[1]无锡市传染病医院感染科,江苏无锡214000

出　　处：《中华医院感染学杂志》2021年第12期1875-1880,共6页Chinese Journal of Nosocomiology

基　　金：江苏省科研基金资助项目(2019141001)。

摘　　要：目的探讨血浆肿瘤坏死因子-α(TNF-α)及其基因多态性与人类免疫缺陷病毒(HIV)感染患者抗病毒治疗后CD^(+)_(4)T细胞水平的相关性。方法选取2015年1月-2019年12月无锡市传染病医院感染科收治的HIV感染患者97例(HIV感染组)和体检的健康志愿者(对照组)30名为研究对象。检测受试者血浆TNF-α水平和血清CD^(+)_(4)细胞水平;多重SnaPshot方法检测全血DNA样本TNF-α基因的多态性。结果HIV感染组血浆TNF-α高于对照组,而治疗前CD^(+)_(4)T细胞低于对照组(P<0.001)。TNF-α基因多态性以rs1800629(包含野生纯合子GG、突变纯合子AA、突变杂合子GA)和rs361525(包含野生纯合子GG、突变杂合子GA基因)、rs1800630(包含野生纯合子CC、突变纯合子AA、突变杂合子CA基因)三个位点基因较为常见。HIV感染组TNF-α位点rs1800629基因型为GA的分布频率和等位基因为A的分布频率高于对照组(P<0.05)。非条件Logistic回归分析结果显示TNF-α基因rsl800629位点中,GA基因型频率、A等位基因频率与HIV感染的发病风险相关(P<0.001)。治疗后CD^(+)_(4)T细胞水平在TNF-α位点rs1800629基因型GG、GA和AA频率差异有统计学意义(P<0.001)。A等位基因的治疗后CD^(+)_(4)T细胞水平较G等位基因组的明显低(P<0.001)。结论TNF-αrsl800629位点突变杂合子GA和A等位基因是HIV感染风险和抗逆转录病毒治疗后CD^(+)_(4)T细胞水平恢复的影响因素,有望为HIV感染风险的评估和抗病毒治疗提供可靠依据。OBJECTIVE To investigate the correlation between plasma tumor necrosis factor-α(TNF-α)and its gene polymorphisms and the levels of CD^(+)_(4)T cell in human immunodeficiency virus(HIV)infected patients after antiviral therapy.METHODS From Jan.2015 to Dec.2019,97 HIV-infected patients(HIV-infected group)admitted to the department of Infectious Diseases at Wuxi Infectious Disease Hospital and 30 healthy volunteers(control group)were selected as the research subjects.The plasma TNF-αlevel and serum CD^(+)_(4)cell level of the subjects were detected,and whole blood DNA samples were collected to detect TNF-αgene polymorphisms by multiple SnaPshot method.RESULTS The plasma TNF-αlevel of HIV-infected group was significantly higher than that of the control group,while CD^(+)_(4)T cell level before treatment was significantly lower than that of the control group,(P<0.001).The TNF-αgene polymorphisms were more common in three loci genes,rs1800629(including wild homozygote GG,mutation homozygote AA,and mutation heterozygote GA),rs361525(including wild homozygote GG and mutation heterozygote GA)and rs1800630(including wild homozygote CC,mutation homozygote AA and mutation heterozygote CA).The frequencies of genotype GA and allele A of TNF-αlocus rs1800629 in the HIV-infected group were significantly higher than those in the control group(P<0.05).Results of unconditional Logistic regression analysis showed that in TNF-αgene locus rsl800629,the frequency of GA genotype and A allele were related to the risk of HIV infection(P<0.001).After treatment,the CD^(+)_(4)T cell levels in terms of genotypes GG,GA and AA frequency differences of TNF-αlocus rs1800629 were significant(P<0.001).The CD^(+)_(4)T cell levels in patients carrying A allele were significantly lower than that of patients carrying G allele after treatment(P<0.001).CONCLUSION Mutation heterozygote GA and A allele of TNF-αrsl800629 locus were factors influencing the risk of HIV infection and the recovery of the CD^(+)_(4)T cell level after antiretroviral the

关 键 词：人类免疫缺陷病毒 感染 肿瘤坏死因子-α CD^(+)_(4)T细胞 基因多态性 

分 类 号：R512.91[医药卫生—内科学]