外加固体缓释碳源的两段反硝化工艺脱氮性能  

Two-Stage Denitrification Process Performance with Solid Slow-Release Carbon Source

作　　者：唐义 马邕文 万金泉 王艳 叶刚 TANG Yi;MA Yong-wen;WAN Jin-quan;WANG Yan;YE Gang(School of Environment and Energy,South China University of Technology,Guangzhou 510006,China;Guangdong Engineering and Technology Research Center of Plant Fiber High-value Cleaning and Utilization,Guangzhou 510641,China)

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]广东省植物纤维高值化清洁利用工程技术研究中心,广州510641

出　　处：《环境科学》2021年第7期3392-3399,共8页Environmental Science

基　　金：国家自然科学基金项目(31570586,31670585)。

摘　　要：传统生物脱氮工艺处理废水过程中,出水硝态氮(NO^(-)_(3)-N)浓度过高是导致总氮(TN)超标的主要原因.通过采用外加碳源提高碳氮比(C/N),强化反硝化过程可有效解决这一难题.利用基于两段反硝化工艺原理研发设计的一体化脱氮反应器,通过在第二段反硝化池投加聚丁二酸丁二醇酯(PBS),采用扫描电子显微镜,对反硝化过程前后PBS材料进行表征,采用扩增子测序,深入探究投加PBS前后,第二段反硝化池中微生物群落结构变化.连续120 d的运行数据表明,COD去除率从95.7%降到90.8%,总氮去除率从51.8%提高到80%,Proteobacteria菌门相对丰富度从36.1%上升到46.1%,Thermomonas菌属相对丰富度从6.47%上升到13.48%.结果表明,投加PBS后,PBS不仅可以为反硝化作用提供碳源,其表面还可作为微生物生长附着的载体,起到良好的挂膜作用,同时提高了反硝化菌丰富度,强化了反硝化过程,脱氮性能明显增强,有效提高系统总氮去除率.During wastewater treatment using a traditional biological denitrification process,the excessive concentration of nitrate nitrogen( NO^(-)_(3)-N) in the effluent is the primary cause of excessive total nitrogen( TN) generation. By using an external carbon source to increase the carbon to nitrogen ratio( C/N),the denitrification process can be strengthened,which effectively addresses this problem. Using an integrated denitrification reactor developed based on the two-stage denitrification process principle with the addition of polybutylene succinate( PBS) in the second stage,the denitrification process was analyzed using a scanning electron microscope before and after characterization of PBS materials. Moreover,amplicon sequencing was used for in-depth exploration of changes in the microbial community structure in the second denitrification pool before and after the addition of PBS. The data of a continuous 120-day experiment showed that the COD removal rate dropped from 95. 7% to 90. 8%,the TN removal rate increased from 51. 8% to 80%,the relative abundance of Proteobacteria phylum rose from 36. 1% to 46. 1%,and the relative abundance of Thermomonas rose from 6. 47%to 13. 48%. The results show that after the addition of PBS,PBS can not only provide carbon source for denitrification,but its surface can also serve as a carrier for microbial growth and attachment,play a good role in filming,and increase the abundance of denitrifying bacteria and strengthen denitrification. During the nitrification process, denitrification performance was significantly enhanced,effectively improving the TN removal rate of the system.

关 键 词：两段反硝化 碳氮比 固体缓释碳源 脱氮 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]