盆腔炎患者SULT1A1基因多态性及其机制  

SULT1A1 gene polymorphism of patients with pelvic inflammation and its mechanism

作　　者：郑朗 胡春艳[1] 李玮 刘俊[1] 陈春英[1] ZHENG Lang;HU Chun-yan;LI Wei;LIU Jun;CHEN Chun-ying(Hainan Provincial People's Hospital(Hainan Hospital Affiliated to Hainan Medical College),Haikou,Hainan 570311,China)

机构地区：[1]海南省人民医院(海南医学院附属海南医院)妇科,海南海口570311

出　　处：《中华医院感染学杂志》2021年第11期1716-1720,共5页Chinese Journal of Nosocomiology

基　　金：海南省自然科学基金面上基金资助项目(817362)。

摘　　要：目的探讨盆腔炎症性疾病(PID)的易感性与磺基转移酶A1基因(SULT1A1)及其机制。方法选择海南省人民医院妇科2019年1月-2020年1月收治的盆腔炎患者100例作为研究组,选择同期医院进行体检的健康女性100名作为对照组。采用聚合酶链式扩增反应(PCR)检测SULT1A1基因rs9282861位点多态性,采用酶联免疫吸附法(ELISA)检测血清中SULT1A1、雌二醇(E2)、促卵泡成熟激素(FSH)水平。结果两组研究对象SULT1A1基因rs9282861位点携带GG、GA基因型及G/A等位基因频率差异无统计学意义,研究组PID患者AA基因型频率(12.00%)高于对照组(P<0.05);校正Logistic回归分析结果表明,携带AA基因型为PID发生的危险因素(P<0.05);PID不同感染严重程度患者SULT1A1基因rs9282861位点携带基因型频率比较差异无统计学意义;PID患者SULT1A1基因rs9282861位点携带不同基因型患者血清SULT1A1、E2、FSH水平差异有统计学意义(P<0.05),SULT1A1水平GG型<GA型<AA型,E2、FSH水平GG型>GA型>AA型,差异均有统计学意义(P<0.05)。结论 SULT1A1基因多态性与本地区女性盆腔炎易感性有关,AA型易感性较高,其机制可能与SULT1A1影响雌激素水平有关。OBJECTIVE To investigate the susceptibility of pelvic inflammatory disease(PID),sulfotransferase A1 gene(SULT1 A1)and its mechanism.METHODS Totally 100 patients with pelvic inflammatory disease admitted to the Department of Gynecology,Hainan Provincial People′s Hospital from Jan.2019 to Jan.2020 were selected as the research group,and 100 healthy women who underwent physical examinations in the hospital during the same period were selected as the control group.Polymerase chain amplification reaction(PCR)was used to detect the polymorphism of SULT1 A1 gene rs9282861 locus,and enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of SULT1 A1,estradiol(E2),and follicle-stimulating hormone(FSH).RESULTS There was no significant difference in the frequency of GG,GA genotypes and G/A alleles carried at the rs9282861 locus of SULT1 A1 gene between the two groups of subjects.The frequency of AA genotypes in PID patients in the study group(12.00%)was significantly higher than that in the control group(P<0.05)The adjusted logistic regression analysis showed that carrying the AA genotype was a risk factor for the occurrence of PID(P<0.05);there was no significant difference in the frequency of SULT1 A1 gene rs9282861 carrying genotype in PID patients with different severity infection;SULT1 A1 gene in PID patients There was a significant difference in serum SULT1 A1,E2 and FSH levels of patients with different genotypes at rs9282861 locus(P<0.05).SULT1 A1 level of GG type was lower than GA type,and less than AA type.E2,FSH level of GG type was significantly lower than GA type,and less than AA type(P<0.05).CONCLUSION SULT1 A1 gene polymorphism was related to the susceptibility of female pelvic inflammatory disease in this area,and the susceptibility of AA type was higher.The mechanism might be related to the influence of SULT1 A1 on the level of estrogen.

关 键 词：磺基转移酶 基因多态性 盆腔炎 雌激素 易感性 

分 类 号：R711.33[医药卫生—妇产科学]