康复训练介导NGF/VEGF/CD34表达对脑缺血再灌注大鼠血管再生的影响  

Effect of rehabilitative training on vascular regeneration in rats with cerebral ischemia-reperfusion through NGF/VEGF/CD34 pathway

作　　者：何文娟[1] 尹伟英[1] 魏妮[1] 谢辉[1] He Wenjuan;Yin Weiying;Wei Ni(Department of Rehabilitation,Chenzhou First People’s Hospital,Chenzhou 423000,China)

机构地区：[1]郴州市第一人民医院康复科,湖南郴州423000

出　　处：《中国康复》2021年第7期387-391,共5页Chinese Journal of Rehabilitation

摘　　要：目的:观察康复训练对脑缺血再灌注大鼠神经功能的影响,探讨康复训练调节血管再生的机制。方法:将SD大鼠适应性喂养1周后,进行脑缺血再灌注手术造模,24h后挑选神经功能评分2~3分的大鼠和假手术大鼠分为假手术组(8只),康复组(16只)和脑缺血组(24只),康复组每天按计划进行跑步训练,分别于康复训练的第7天、第14天对大鼠进行神经行为学评分并随机挑选部分大鼠进行取材进行TTC染色、免疫组化及免疫印迹实验。结果:脑缺血再灌注造成大鼠神经功能损伤,7d和14d的康复训练有效提高了大鼠神经行为学评分(P<0.05)。此外,脑缺血再灌注诱导了大鼠脑中血管内皮生长因子(VEGF)与神经生长因子(NGF)蛋白表达增加,促进脑中血管微循环的建立;与脑缺血组相比,7d和14d康复训练显著增加了大鼠脑中VEGF与NGF表达(P<0.05),加速了缺血区血管微循环的建立(P<0.05)。结论:康复训练对于脑缺血再灌注有良好的治疗效果,其机制可能是增加了VEGF与NGF表达,加速了缺血区血管微循环的建立。Objective:To observe the effects of rehabilitative training on neuronal function in rats with cerebral ischemia reperfusion(I/R),and to explore the mechanism of rehabilitative training on vascular regeneration.Methods:After one week of adaption,cerebral I/R surgeries were performed on SD rats.After 24 h,I/R rats with neurological scores of 2-3 points were divided into cerebral I/R group(24 animals)and rehabilitative training group(16 animals).A total of,8 rats receiving sham operation served as control group.The rats in rehabilitative training group exercised according to the procedure every day.On the 7th and 14th day,rats were scored for neuronal function and some rats were randomly selected for TTC staining,immunohistochemistry and immunoblotting experiments.Results:I/R caused neurological damage in rats.Rehabilitative training on the 7th and 14th day effectively improved the neurological scores(P<0.05).In addition,I/R promoted the expression of vascular endothelial growth factor(VEGF)and nerve growth factor(NGF)proteins in the rat brain and promoted the establishment of vascular microcirculation in the brain.As compared with cerebral I/R group,rehabilitative training on the 7th and 14th day significantly increased the expression of VEGF and NGF(P<0.05)and accelerated the establishment of vascular microcirculation in infarct area(P<0.05).Conclusion:Rehabilitative training can effectively improve the neuronal functions which were destroyed due to cerebral I/R in rats probably by accelerating the establishment of vascular microcirculation in the ischemic area by increasing the expression of VEGF and NGF.

关 键 词：脑缺血再灌注损伤 康复训练 神经功能障碍 血管微循环 

分 类 号：R49[医药卫生—康复医学] R743.3[医药卫生—临床医学]