应用优化的3T3细胞中性红摄取试验评价6种化妆品的光毒性  

Evaluation of Phototoxicity of 6 Cosmetic Products by Optimized 3T3 Cell Neutral Red Uptake Test

作　　者：段名扬 康桦 桑晶 匡荣 DUAN Mingyang;KANG Hua;SANG Jing;KUANG Rong(Collaboration Innovation Center of Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China;NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics,Hangzhou 310052,China)

机构地区：[1]浙江工业大学绿色制药协同创新中心,杭州310014 [2]国家药品监督管理局化妆品动物替代试验技术重点实验室,杭州310052

出　　处：《中国现代应用药学》2021年第11期1309-1313,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技计划项目(2018C37122);国家药品监督管理局化妆品动物替代试验技术重点实验室(国药监科外函[2019]82号)。

摘　　要：目的建立一种针对化妆品的体外光毒性检测方法。方法选取6批市售化妆品样品,通过摸索样品的细胞毒性,确定光毒浓度区间,设立样品加阳性对照的参比物,再进行光毒性评价,通过Phototox软件分析结果。结果6种市售化妆品光刺激因子≤2,表明均无潜在光毒性。建立适合于检测化妆品的样品阳性对照,且与阳性对照结果均表现强光毒性。结论该方法初步具备对化妆品光毒性评价的可行性,适合于中国国情的体外方法标准。OBJECTIVE To establish an in vitro phototoxicity detection method for cosmetic products.METHODS Six batches of cosmetic samples sold in the market were selected.The phototoxicity concentration range was determined by exploring the cytotoxicity of the samples.Reference substances including samples and positive controls were set up and the phototoxicity was evaluated.The results were analyzed by Phototox software.RESULTS The photo irritation factor of 6 kinds of cosmetics sold in the market was≤2,which indicated that all of them had no potential phototoxicity.The sample positive control of samples suitable for the detection of products was established.The results of sample positive control and positive control showed strong phototoxicity.CONCLUSION This method has the feasibility of evaluating the phototoxicity of cosmetics and innovates the in vitro method standard which is suitable for the situation of our country.

关 键 词：3T3细胞 化妆品 光毒性 

分 类 号：R917.101[医药卫生—药物分析学]