新鲜羊膜对兔宫腔黏连的预防作用及对子宫内膜容受性的改善作用  

Preventive effect of fresh amniotic membrane on intrauterine adhesion and its improvement effect on endometrial receptivity in rabbits

作　　者：范艳艳[1] 王娜[1] 徐文[1] 葛亚杰 王丽娜[1] FAN Yanyan;WANG Na;XU Wen;GE Yajie;WANG Lina(Department of Gynecology,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院普通妇一科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2021年第4期990-998,共9页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目(20180414061GH);吉林省财政厅卫生专项项目(JLSWSRCZX2020-061)。

摘　　要：目的:建立兔宫腔黏连(IUA)模型,探讨宫腔内放置新鲜羊膜预防IUA的效果和对子宫内膜容受性的改善作用。方法:雌性新西兰大白兔30只随机分为对照组、模型组和治疗组,每组10只,模型组和治疗组兔用刮勺搔刮宫腔机械性损伤子宫内膜并于宫腔内放置浸泡脂多糖(LPS)的棉线使宫腔感染,建立兔IUA模型,治疗组兔于模型建立同时宫腔内放置新鲜羊膜,对照组为假手术组,不进行宫腔处理。收集建模后第1和2周时的子宫标本,采用HE染色和Masson染色检测各组兔子宫内膜组织中腺体数量和子宫内膜纤维化面积百分率,采用免疫组织化学染色法检测各组兔子宫内膜组织中整合素αVβ3和白血病抑制因子(LIF)蛋白表达水平。结果:与对照组比较,第1和2周时模型组和治疗组兔子宫内膜组织中腺体数量明显减少(P<0.05),子宫内膜纤维化面积百分率明显升高(P<0.05),子宫内膜组织中整合素αVβ3和LIF蛋白表达水平明显降低(P<0.05);与模型组比较,第1和2周时治疗组兔子宫内膜组织中腺体数量明显增加(P<0.05),子宫内膜纤维化面积百分率明显降低(P<0.05),子宫内膜组织中整合素αVβ3和LIF蛋白表达水平明显升高(P<0.05);与第1周时比较,第2周时对照组和治疗组兔子宫内膜组织中腺体数量、子宫内膜纤维化面积百分率及子宫内膜组织中整合素αVβ3和LIF蛋白表达水平差异均无统计学意义(P>0.05),但第2周时模型组兔子宫内膜纤维化面积百分率明显降低(P<0.01),子宫内膜组织中整合素αVβ3和LIF蛋白表达水平明显升高(P<0.05)。结论:宫腔内放置新鲜羊膜可以减轻IUA的程度,改善子宫内膜容受性,且起效快并作用持久。Objective:To set up the rabbit intrauterine adhesion(IUA)models,and to explore the effect of placing fresh amniotic membrane in the uterine cavity in the prevention of IUA and its improvement effect on the endometrial receptivity.Methods:Thirty female New Zealand white rabbits were randomly divided into control group,model group and treatment group,with 10 in each group.Using scraping intrauterine mechanical damage to the endometrium and placing cotton lines soaked in lipid polyaccharides(LPS)in the intrauterine cavity,the rabbit IUA models were established in model group and treatment group.For the rabbits in treatment group,fresh amniotic membrane was placed in the uterine cavity at the same time.The control group was a sham operation group with no uterine treatment.The uterine specimens were collected at the 1st and 2nd weeks.The number of glands and the percentages of endometrial fibrosis areas in endometrium tissue of the rats rabbits in various groups were compared by HE and Masson staining,and the expression levels of integrinαVβ3 and leukemia inhibitory(LIF)proteins in endometrium tissue of the rabbits were detected by immunohistochemical staining.Results:Compared with control group,the number of glands in endometrium tissue of the rabbits in model group and treatment group were decreased significantly at the lst and 2nd weeks(P<0.05),the percentages of endometrial fibrosis areas were increased significantly(P<0.05),and the expression levels of integrinαVβ3 and LIF proteins in endometial tissue were decreased significantly(P<0.05).Compared with model group,the number of glands in endometrium tissue of the rabbits in treatment group was increased significantly at the 1st and 2nd weeks(P<0.05),the percentages of the endometrial fibrosis areas were decreased significantly(P<0.05),and the expression levels of integrinαVβ3 and LIF proteins in endometrium tissue were increased significantly(P<0.05).In control group and treatment group,compared with the 1st week,there were no statistical differences in the

关 键 词：宫腔黏连 新鲜羊膜 整合素ΑVΒ3 白血病抑制因子 子宫内膜容受性 

分 类 号：R711.74[医药卫生—妇产科学]