氮磷氯共掺杂碳点荧光探针用于食品中亚铁氰离子的检测  

Nitrogen,phosphorus and chlorine co-doped carbon dots as fluorescence nanoprobe for the determination of ferrocyanide ions in food stuffs

作　　者：张简 钱敏捷 陈慧敏 楚玉芹 杨振泉[1] 肖丽霞[1] 胡钦 ZHANG Jian;QIAN Min-jie;CHEN Hui-min;CHU Yu-qin;YANG Zhen-quan;XIAO Li-xia;HU Qin(College of Food Science and Engineering,Yangzhou University,Yangzhou 225001,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225001

出　　处：《化学研究与应用》2021年第7期1251-1259,共9页Chemical Research and Application

基　　金：江苏省基础研究计划(自然科学基金)青年基金项目(BK20200949)资助;2019年度扬州市绿扬金凤计划项目资助;2020年江苏省大学生创新创业训练计划项目(202011117082Y)资助;江苏省高等学校自然科学研究面上项目(19KJB150042)资助;中国博士后科学基金面上项目(2020M671625)资助;2020年度扬州市重点研发计划(社会发展)项目(YZ2020063)资助;2019年度扬州大学科技创新培育基金项目(2019CXJ188)资助;江苏省乳业生物工程技术研究中心开放课题(KYRY2019002)资助。

摘　　要：本工作采用酸碱中和自放热法,以葡萄糖为碳源,乙二胺、磷酸、盐酸为杂原子供体,制备氮磷氯共掺杂碳点(N,P,Cl-CDs)。基于亚铁氰离子(FeCNs)可以有效猝灭N,P,Cl-CDs荧光,建立了一种快速灵敏检测FeCNs的荧光方法。在最佳实验条件下,N,P,Cl-CDs对FeCNs检测展现出高选择性和高灵敏度,FeCNs浓度在0.1~10.0和10.0~70.0μg·mL^(-1)范围内与N,P,Cl-CDs荧光猝灭效率呈现良好的线性关系,检测限分别低至为8.4和19.8 ng·mL^(-1)。最后,本工作将该方法用于食品样品中FeCNs的检测,加标回收率为95.8%~105.0%,相对标准偏差低于5.67%,结果令人满意。In this work,a nitrogen,phosphorus and chlorine co-doped carbon dots(N,P,Cl-CDs)was prepared via an acid-base neutralization self-exothermic method with glucose served as the carbon source,and ethylenediamine,phosphoric acid and hydrochloric acid as heteroatom donors.Based on the observation that the ferrocyanide ions(FeCNs)could effectively quench the fluorescence of N,P,Cl-CDs,a rapid and sensitive fluorescence method was established for FeCNs detection.Under the optimal experimental conditions,the N,P,Cl-CDs showed high selectivity and sensitivity towards FeCNs detection with a good linear relationship between the FeCNs concentration and the fluorescence quenching efficiency was achieved in the concentration ranges of 0.1~10.0 and 10.0~70.0μg·mL^(-1),and the corresponding detection limit was as low as 8.4 and 19.8 ng·mL^(-1),respectively.Finally,the proposed fluorescence method was used for FeCNs detection in food samples with satisfactory results.The quantitative spiked recoveries were in a range of 95.8%~105.0% with the relative standard deviation less than 5.67%.

关 键 词：氮磷氯掺杂碳点 亚铁氰离子 荧光猝灭 

分 类 号：O657.3[理学—分析化学]