餐厨固渣酶法制备适用于污水处理的外加碳源  

Preparation of external carbon source suitable for wastewater treatment from food-waste solid residue via enzymatic hydrolysis

作　　者：赵淑兰 占洁 王涛[1] 廖家林[1] 赵明星[1] 黄振兴[1,3] ZHAO Shulan;ZHAN Jie;WANG Tao;LIAO Jialin;ZHAO Mingxing;HUANG Zhenxing(School of Environmental and Civil Engineering,Jiangnan University,Wuxi 214122,China;Wuxi Hengcheng Water Conservancy Engineering Construction Co.Ltd.,Wuxi 214000,China;Jiangsu Cooperative Innovation Center of Technology and Material of Water Treatment,Suzhou 215009,China)

机构地区：[1]江南大学环境与土木工程学院,江苏无锡214122 [2]无锡恒诚水利工程建设有限公司,江苏无锡214000 [3]江苏省水处理技术与材料协同创新中心,江苏苏州215009

出　　处：《食品与发酵工业》2021年第14期145-150,159,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2019YFC1906302);国家自然科学基金面上项目(51678279);江苏省重点研发项目(BE2020755)。

摘　　要：餐厨垃圾中含有大量有机碳源,具有较好的资源化利用属性。采用酶法从餐厨固渣中回收和制备适用于污水处理的外加碳源。通过考察酶解条件对碳源提取效果的影响及关键因子间的交互关系,以明确最佳酶解条件。在此基础上,借助等电点沉淀法进一步提高回收碳源纯度。结果表明,相较于单一淀粉酶,α-淀粉酶和γ-淀粉酶协同处理可以提高碳源提取效果,并能减少酶使用量。酶解温度、pH和底物质量浓度是影响复合淀粉酶作用效果的关键因素,对碳源提取率影响大小依次为底物质量浓度>温度>pH,且底物质量浓度与温度交互作用显著。在温度57.0℃、pH 6.2、底物质量浓度190 g/L,酶添加质量分数0.6%以及复合酶质量比3∶1(α∶γ)的最佳酶解条件下,碳源提取率达到76.9%。经等电点沉淀处理后,碳源回收液中生化需氧量(biochemical oxygen demand,BOD)质量浓度和BOD/N值分别达到了76.8 g/L和51.2,非常有作为污水处理碳源使用的潜力。There are a lot of organic carbon sources in food waste,which shows a good property of resource utilization.By enzymatic hydrolysis,the carbon source suitable for wastewater treatment was recycled and prepared from food-waste solid residue.To acquire the optimal enzymatic conditions,the influence of enzymatic conditions on carbon source extraction and the interaction between key factors were investigated.Subsequently,isoelectric precipitation method was used to further improve the purity of recovered carbon source solution.The results demonstrated that the co-treatment ofα-amylase andγ-amylase improved the extraction of carbon source and could reduce the enzyme amount used.Enzymatic temperature,pH and substrate mass concentration were the key factors affecting the synergistic effect of the two amylases.The order of influences on the extraction efficiency of carbon source was substrate mass concentration>temperature>pH,and there was a significant interaction effect between substrate mass concentration and temperature.The optimal conditions for carbon source extraction were 57.0℃,pH 6.2,substrate mass concentration 190 g/L,enzyme mass fraction added 0.6%,enzyme mass ratio 3∶1(α∶γ),where the extraction efficiency of carbon source reached 76.9%.By isoelectric precipitation,the finally biochemical oxygen demand(BOD)concentration and BOD/N ratio in the recovered solution of carbon source reached 76.8 g/L and 51.2,respectively.The recovered solution can be used as a promising carbon source for wastewater treatment.

关 键 词：餐厨固渣 淀粉酶 碳源 生化需氧量 等电点沉淀 

分 类 号：X703[环境科学与工程—环境工程] X799.3