补肾健骨方含药血清对ROBs和rBMSCs增殖、分化和矿化的影响  

Effect of nourishing kidney and strong bone recipe-containing serum on the proliferation and differentiation of osteoblasts and bone marrow mesenchymal stem cells in rats

作　　者：周湘琳 王冬含 施根根 赵良功[2] 封士兰[1] ZHOU Xianglin;WANG Donghan;SHI Gengen;ZHAO Lianggong;FENG Shilan(School of Pharmacy,Lanzhou University,Lanzhou 730000;The Second Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]兰州大学第二医院,甘肃兰州730000

出　　处：《中国骨质疏松杂志》2021年第7期958-963,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81703664);中央高校基本科研业务费专项资金资助(lzujbky-2018-57)。

摘　　要：目的观察补肾健骨方含药血清对大鼠颅骨成骨细胞(rat calvarial osteoblasts,ROBs)和大鼠骨髓间充质干细胞(rat bone marrow mesenchymal stem cells,r BMSCs)细胞活性及分化、矿化的影响。方法制备补肾健骨方含药血清,将ROBs和r BMSCs分为5%、10%、15%、20%、25%浓度含药血清组、无药血清组及传统诱导组,分别对各组ROBs和r BMSCs进行成骨诱导。MTT法检测ROBs和r BMSCs细胞的增殖情况;用试剂盒检测ROBs和r BMSCs的碱性磷酸酶(ALP)活性;茜素红染色法检测矿化结节形成。结果不同浓度含药血清组与空白无药血清组、传统诱导组相比,均不同程度地促进ROBs和r BMSCs的增殖,使ALP活性提高,增加钙化结节数量和面积。其中含药血清浓度为20%时促进ROBs增殖分化作用最佳,浓度为10%时促进r BMSCs增殖分化效果最为显著。结论不同浓度补肾健骨方含药血清均能够促进ROBs和r BMSCs的成骨增殖、分化和矿化,分别在浓度为20%和10%时最佳。Objective To observe the effect of nourishing kidney and strong bone recipe-containing serum on the proliferation and differentiation of rat calvarial osteoblasts(ROBs)and bone marrow mesenchymal stem cells(rBMSCs).Methods The nourishing kidney and strong bone recipe-containing serum was prepared.ROBs and rBMSCs were divided into blank serum group,5%,10%,15%,20%,and 25%concentration drug-containing serum group,and traditional induction group.MTT method was used to detect the proliferation of ROBs and rBMSCs.Alkaline phosphatase(ALP)kit was used to detect the ALP activity of ROBs and rBMSCs.Alizarin red staining was used to detect the formation of mineralized nodules.Results Compared with those in the blank serum group and the traditional induction group,the drug-containing serum groups at different concentrations promoted the proliferation of ROBs and rBMSCs to different degrees,increased the ALP activity,and increased the number and area of calcified nodules(P<0.05).Among them,the effect of promoting the proliferation and differentiation of ROBs was the best at the concentration of 20% of drug-containing serum.The effect of promoting the proliferation and differentiation of rBMSCs was the best at the concentration of 10% of drug-containing serum.Conclusion The nourishing kidney and strong bone recipe-containing serum promotes the osteogenic proliferation and differentiation of ROBs and rBMSCs at the concentration of 20% and 10%.,respectively.

关 键 词：中医中药 含药血清 大鼠颅骨成骨细胞 大鼠骨髓间充质干细胞 细胞增殖 成骨分化 

分 类 号：R289.9[医药卫生—方剂学] K332[医药卫生—中药学]