艾灸督脉改变AD转基因小鼠事件相关电位P300的分析研究  

Analysis and Study of Moxibustion on Governor Vesse on AD Transgenic Mice Event-related Potential P300

作　　者：钟倩 朱才丰[2] 宋小鸽[3] 杨骏[4] 潘洪萍[2] 贺成功[2] 杨坤[2] 秦晓凤[2] ZHONG Qian;ZHU Cai-feng;SONG Xiao-ge;YANG Jun;PAN Hong-ping;HE Cheng-gong;YANG Kun;QIN Xiao-feng(School of Graduate,Anhui University of Chinese Medicine,Hefei 230038,China;The Third Department of Geriatrics,The Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,China;Institute of Acupuncture and Meridian,Anhui University of Chinese Medicine,Hefei 230038,China;Department of Acupuncture and Rehabilitation,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学研究生院,合肥230038 [2]安徽中医药大学第二附属医院老年病三科,合肥230061 [3]安徽中医药大学针灸经络研究所,合肥230038 [4]安徽中医药大学第一附属医院针灸康复科,合肥230038

出　　处：《江西中医药大学学报》2021年第4期98-101,共4页Journal of Jiangxi University of Chinese Medicine

基　　金：国家自然科学基金项目(81603701);安徽省高校自然科学研究项目(KJ2016A416);安徽省卫计委中医药科研计划项目(2016zy05);安徽高校科研平台建设项目(2015TD033)。

摘　　要：目的:通过艾灸督脉分析AD转基因小鼠事件相关电位P300的变化。方法:AD转基因小鼠随机分为模型组、艾灸组、西药Ⅰ组、西药Ⅱ组,选取同龄同背景C57BL/6J雄性小鼠作为正常组,每组10只。艾灸组小鼠隔附子饼灸"百会",温和灸"天门(风府)""大椎",每次20 min,1次/d,治疗2 w;西药Ⅰ组用雷帕霉素腹腔注射给药,2 mg·kg^(-1)·d^(-1),1次/d,治疗2 w;西药Ⅱ组在艾灸组治疗同时予以3-甲基腺嘌呤(3-MA)腹腔注射给药,1.5 mg·kg^(-1)·d^(-1),1次/d,治疗2 w;正常组和模型组动物常规饲养2 w,不作特殊处理。在实验前后分别测定各组P300,将测定结果进行比较。结果:治疗前正常组与模型组、艾灸组、西药Ⅰ组、西药Ⅱ组的N2潜伏期、P3潜伏期、P3波幅相比较差异均有统计学意义(P<0.05),AD小鼠的N2潜伏期、P3潜伏期较正常小鼠延长,P3波幅降低;艾灸组与西药Ⅰ组在治疗后N2潜伏期较治疗前无明显变化(P>0.05),P3潜伏期与治疗前缩短,P3波幅升高(P<0.05);艾灸组与西药Ⅰ组在治疗后比较P3潜伏期、P3波幅无差异(P>0.05);西药Ⅱ组治疗前后比较N2潜伏期、P3潜伏期、P3波幅均无差异(P>0.05)。结论:阿尔茨海默病小鼠认知功能与P300的特征相一致;艾灸督脉能提高缩短AD小鼠P3潜伏期、升高P3波幅,是治疗AD的有效方法。Objective:To observe the effect of moxibustion on governor vessel on event-related potentials P300 in mice with AD transgenic.Methods:APP/PS1 Double-transgenic mice were randomly divided into Moxibustion group,MedicationⅠgroup,MedicationⅡgroup,and Model group;C57 BL/6 J female mice(the same age and the samebackground)were used as the normal group(10 in each group).Moxibustion(separated by Radix Aconiti Praeparata cake)on"Baihui"(GV 20),"Tianmen"(GV 16)and"Dazhui"(GV 14)for 20 min,once daily for 2 weeks,with one day’s off between two weeks.The mice of the MedicationⅠgroup were injected with Rapamycin(2 mg·kg^(-1)·d^(-1),q.d)by lavage for 2 weeks.Accepted the treatment in moxibustion group,the mice of the MedicationⅡgroup were also given with 3-MA peritoneal injection(1.5 mg·kg^(-1)·d^(-1),q.d)by lavage for 2 weeks.The mice of the normal group and model group were routinely fed for 2 weeks.Each group of P300 was measured before and after the experiment,and the results were compared.Results:The differences between N2 incubation period,P3 incubation period and P3 amplitude of the normal group and model group before treatment were statistically significant(P<0.05);The N2 incubation period and P3 incubation period were longer than in normal mice in AD mice,and the P3 amplitude was reduced;There was no significant difference between the moxibustion group and western medicine groupⅠin N2 incubation period after treatment compared with that before treatment(P>0.05),P3 latency and pretreatment shortened,P3 amplitude increased(P<0.05);moxibustion group and Western medicine GroupⅠcompared P3 incubation period and P3 wave extent with no difference after treatment(P>0.05);There was no difference in N2 latency,P3 latency and P3 amplitude between western medicine groupⅡbefore and after treatment(P>0.05).Conclusion:The cognitive function of alzheimer’s disease mice is consistent with the characteristics of P300;Moxibustion on the vessel can shorten the incubation period and increase the amplitude of P300 in

关 键 词：阿尔茨海默病 P300测定 认知功能 事件相关电位 艾灸 

分 类 号：R245[医药卫生—针灸推拿学]