Isolation of a Pseudomonas Stutzeri strain that degrades 1,2,4-trichlorobenzene and characterization of its degradative plasmid  

作　　者：Lei SONG Hui WANG Hanchang SHI Hongying HU 

机构地区：[1]Environmental Simulation and Pollution Control State Key Joint Laboratory,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Frontiers of Environmental Science & Engineering》2008年第1期69-72,共4页环境科学与工程前沿（英文）

基　　金：This work was supported by the National High Technology Research and Development Program of China(863 Program)(Grant Nos.2002AA601170 and 2002A A601150).

摘　　要：The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic plasmids.The aim of the present work was to know the location of the genes for degrading 1,2,4-trichlorobenzen.In this paper a 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin Chemical Plant using 1,2,4-trichlorobenzene as the sole carbon source.The strain was identified as Pseudomonas stutzeri through morphologic survey and 16S rDNA sequence determination.A plasmid was discovered from strain THSL-1 by using the alkali lysis method.When the plasmid was transformed into E.coli.JM109 by the CaCl2 method,the transformant could grow using 1,2,4-trichlorobenzene as the sole carbon source and had the degradation function of 1,2,4-trichlorobenzene.Therefore,it could be deemed that the plasmid carried the degradative genes of 1,2,4-trichlorobenzene.The average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes(HindIII,BamHI,and XholI)for single cutting and double cutting the plasmid pTHSL-1,respectively.

关 键 词：1 2 4-TRICHLOROBENZENE 16S rDNA degradative plasmid Pseudomonas sp. 

分 类 号：Q78[生物学—分子生物学]