Improvement of detection method of Cryptosporidium and Giardia in reclaimed water  

作　　者：Tong ZHANG Xing XIE Hongying HU Yudong SONG Qianyuan WU Zusheng ZONG 

机构地区：[1]Environmental Simulation and Pollution Control State Key Joint Laboratory,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Frontiers of Environmental Science & Engineering》2008年第3期380-384,共5页环境科学与工程前沿（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.20510076);National Natural Science Foundation-Japan Science and Technology Agency Joint-Project(Grant No.20477021).

摘　　要：Cryptosporidium and Giardia are two typical species of pathogenic protozoans that seriously endanger water quality.Previous works indicated that detection of Cryptosporidium and Giardia with modified United States Environmental Protection Agency(USEPA)method-1623 using a membrane filtration-elution for sample concentration attained better recovery and lower cost compared to the USEPA method-1623.Several improvements of membrane filtration-elution step as well as immunomagnetic separation(IMS)steps were investigated and an optimized method for detection of Cryptosporidium and Giardia in wastewater reclamation system was recommended in this paper.The experimental results show that an overnight soak of the membrane after scraping and vortex agitation before elution could enhance and stabilize the recovery.Increasing turbidity to 4 NTU by adding kaolin clay before filtration could effectively improve the recovery of low-turbidity water.Washing the concentrate after centrifugation and twice acid dissociation both reduced the impact of water quality to protozoan recovery.Protozoans in different water samples were determined by this optimized method,and the recovery of Cryptosporidium and Giardia were above 70% and 80%respectively,much higher than the acceptance of method-1623.

关 键 词：CONCENTRATION CRYPTOSPORIDIUM GIARDIA immunomagnetic separation(IMS) wastewater reuse 

分 类 号：X70[环境科学与工程—环境工程]