支气管哮喘患儿外周血调节性T淋巴细胞水平IL-10 sIgE水平及其临床意义  

Levels and Clinical Significance of Peripheral Blood Regulatory T Lymphocytes IL-10 and SIgE in Children with Bronchial Asthma

作　　者：王汝琼 李小明 林道炯 吴守业 周琼花 WANG Ruqiong;LI Xiaoming;LIN Daojiong(Hainan Women's and Children's Medical Center,Hainan Haikou 570000,China)

机构地区：[1]海南省妇女儿童医学中心,海南海口570000

出　　处：《河北医学》2021年第7期1127-1131,共5页Hebei Medicine

基　　金：海南省卫生计生行业科研项目,(编号:18A200077)。

摘　　要：目的:通过对户尘螨过敏的支气管哮喘患儿外周血调节性T淋巴细胞水平、血清白细胞介素-10(IL-10)和特异性IgE(sIgE)水平检测,以探讨其在临床上的指导意义。方法:选取2018年2月至2020年2月我院收治的163例户尘螨过敏的支气管哮喘患儿作为研究对象,根据病情严重程度将其依次分为轻度、中度和重度,其中每组分别为52例、63例和48例,另外选取同期接受体检的健康同龄儿童50例作为对照组,将所有入选者外周血淋巴细胞进行分离后,在户尘螨浸出液的刺激下培养48h,采用流式细胞分析仪检测适应性调节T细胞(iTreg)和I型调节性T细胞(Tr1)所占百分比,另外对各组肺功能状态以及血清IL-10、TGF-β和sIgE水平进行比较。结果:患病组与对照组相比,发现病情程度越严重,第1秒用力呼气容积(FEV1)、第1秒用力呼气量占用力肺活量比值(FEV1/FVC)和呼气峰值流速(PEF)值则越小,iTreg和Tr1细胞所占比例也越小,且差异均具有统计学意义(P<0.05);比较对照组和患病组免疫因子水平,发现病情程度越严重,IL-10和转化生长因子β(TGF-β)水平越低(P<0.05),而sIgE水平则越高(P<0.05)。结论:对支气管哮喘患儿肺功能指标以及分泌IL-10的调节性T淋巴细胞和免疫因子IL-10、TGF-β和sIgE水平的检测,在临床上不仅有助于评估该疾病的严重程度,同时还有利于为疾病治疗过程中的转归提供参考依据。Objective:To explore the clinical guiding significance through detecting the levels of peripheral blood regulatory T lymphocytes,serum interleukin-10(IL-10)and specific IgE(sIgE)in bronchial asthma children allergic to Dermatophagoides pteronyssinus.Methods:From February 2018 to February 2020,163 children with bronchial asthma who were allergic to Dermatophagoides pteronyssinus were selected as study subjects.According to the severity of the disease,they were divided into mild group(52 cases),moderate group(63 cases),and severe group(48 cases).Meanwhile,50 healthy children of the same age were selected as the control group.The peripheral blood lymphocytes were isolated,and then cultured for 48 hours under the stimulation of Dermatophagoides pteronyssinus leachate.Flow cytometry was used to detect the percentages of adaptive regulatory T cells(iTreg)and type I regulatory T cells(Tr1).In addition,the lung function,serum IL-10,TGF-βand sIgE levels were compared among groups.Results:Comparison of the control group and the diseased group found that the severer the disease,the lower the forced expiratory volume in the first second(FEV1),the FEV1 to forced vital capacity(FVC)ratio(FEV1/FVC)and peak expiratory flow(PEF),the lower the proportions of iTreg and Tr1,showing statistically significant differences(P<0.05).Comparison of immune factor levels between the control group and the diseased group found that the severer the disease,the lower IL-10 and TGF-βlevels(P<0.05),and the higher the sIgE level(P<0.05).Conclusion:The detection of lung function indexes,IL-10 secreting regulatory T lymphocytes and immune factors IL-10,TGF-βand SiGe levels in children with bronchial asthma not only helps to evaluate the severity of the disease,but also helps to provide reference for the prognosis of the disease in the process of treatment..

关 键 词：支气管哮喘 调节性T淋巴细胞 白细胞介素-10 特异性IGE 

分 类 号：R725.6[医药卫生—儿科]