养心定悸胶囊在缺血再灌注损伤心肌组织中的保护作用研究  

Protective Effect of Yangxin Dingji Capsule on Myocardial Ischemia Reperfusion Injury

作　　者：王静 贾凌梅 刘畅[2] 栾莹莹 贾敏 WANG Jing(Shijiazhuang People's Hospital,Hebei Shijiazhuang 050027,China)

机构地区：[1]河北省石家庄市人民医院,河北石家庄050027 [2]河北医科大学第二医院心内科,河北石家庄050000

出　　处：《河北医学》2021年第7期1191-1197,共7页Hebei Medicine

基　　金：河北省医学科学研究重点课题计划,(编号:20191487)。

摘　　要：目的:研究养心定悸胶囊对缺血再灌注损伤心肌组织氮代谢的影响。方法:建立缺血再灌注损伤大鼠模型,考察模型大鼠心肌组织乳酸脱氢酶(LDH)及肌酸激酶同工酶(CK)活性,检测抗氧化酶及脂质过氧化产物含量,考察组织中腐胺、精胺、多胺含量,鸟氨酸脱羧酶(ODC)和亚精胺/精胺N-乙酰转移酶活性(SSAT),一氧化氮(NO)及氮自由基含量(ONOO-),检测心肌组织线粒体相关代谢酶活性,并评价养心定悸胶囊干预后的影响。结果:模型大鼠心肌组织乳酸脱氢酶及肌酸激酶同工酶活性显著升高,心肌损伤显著。模型大鼠心肌组织中多胺、腐胺、精胺含量显著升高,ODC和SSAT活性水平显著升高,NO含量显著降低,氮自由基ONOO-明显升高,心肌组织超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性显著降低,硫代巴比妥酸反应物和脂质过氧化物含量显著升高,心肌组织氧化应激反应显著。线粒体代谢酶异柠檬酸脱氢酶、琥珀酸脱氢酶、苹果酸脱氢酶,α-酮戊二酸脱氢酶活性显著降低,说明心肌线粒体能量代谢异常。而养心定悸胶囊干预后,可显著改善心肌损伤,降低心肌损伤指标LDH及CK活性,给与养心定悸胶囊干预后,显著降低ODC和SSAT活性水平,减少多胺,腐胺,精胺含量,氮自由基含量,恢复NO含量水平,改善心肌氮代谢,从而恢复心脏组织抗氧化损伤相关酶活性,减少硫代巴比妥酸反应物和脂质过氧化物含量,并恢复线粒体代谢酶活性。结论:养心定悸胶囊可显著改善缺血再灌注损伤心肌组织氮代谢异常,减少心肌组织胺类物质含量,减少氮自由基,恢复心肌组织能量代谢,从而减少心肌组织损伤的发生。Objective:To study the effect of Yangxindingji Capsule on nitrogen metabolism in myocardial tissue after ischemia-reperfusion injury.Methods:A model of rats with ischemia-reperfusion injury was established.The activities of LDH and CK in the myocardial tissue of the model rats were investigated.The content of antioxidant enzymes and lipid peroxidation products,the content of humic,spermine and polyamine in the tissues,Ornithine decarboxylase(ODC),spermine/spermine N-acetyltransferase activity(SSAT),nitric oxide(no)and nitrogen free radical content(ONOO-)were investigated.The mitochondrial related metabolic enzyme activity of myocardial tissue was detected.The effect of Yangxindingji capsule on the prognosis was evaluated.Results:The activities of LDH and CK isoenzyme in the myocardial tissue of the model rats were significantly increased,and the myocardial damage was significant.The content of polyamine,humic acid and spermine in the myocardial tissue of the model rats was significantly increased,the activity of ODC and SSAT increased,the NO content was significantly decreased,the activity of ONOO-of nitrogen free radical was significantly increased,the activity of superoxide dismutase,catalase and glutathione peroxidase in the myocardial tissue was significantly decreased,and the content of thiobarbituric acid reactant and lipid peroxide increased significantly,The oxidative stress response of myocardial tissue was significant.The mitochondrial metabolic enzymes isocitrate dehydrogenase,succinate dehydrogenase,malate dehydrogenase,The activity ofα-ketoglutarate dehydrogenase was significantly reduced,which indicating that the energy metabolism of mitochondria was abnormal.The results showed that the Yangxindingji capsule could significantly improve the myocardial injury,reduce the LDH and CK activity of myocardial injury index,and give yangxindingji capsule to dry prognosis,significantly reduce the activity level of ODC and SSAT,reduce the content of polyamine,humic,spermine,nitrogen free radical,restore the le

关 键 词：养心定悸胶囊 缺血再灌注损伤 氮代谢 氧化应激损伤 

分 类 号：R285.5[医药卫生—中药学]