溶磷木霉菌对辣椒促生效果  

Effects of Trichoderma Phosphate Solubilizing on the Pepper Growth

作　　者：贺字典 高玉峰 叶雪玲 王玉斌 李婧婷 齐红岩[2] HE Zidian;GAO Yufeng;YE Xueling;WANG Yubin;LI Jingting;QI Hongyan(College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004;College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161;Chengde Agricultural Products Processing Service Center,Chengde,Hebei 067000)

机构地区：[1]河北科技师范学院农学与生物科技学院,河北秦皇岛066004 [2]沈阳农业大学园艺学院,辽宁沈阳110161 [3]承德市农产品加工服务中心,河北承德067000

出　　处：《北方园艺》2021年第13期62-68,共7页Northern Horticulture

基　　金：国家重点研发计划资助项目(2018YFD0201206);河北省重点研发计划资助项目(18226507D);河北省自然科学基金资助项目(C2019407103);河北省高等学校科学技术研究重点资助项目(ZD2019018)。

摘　　要：以Ca_(3)(PO_(4))_(2)为试材,采用溶磷圈法筛选木霉溶磷菌;采用形态学特征和ITS rDNA序列进行木霉溶磷菌鉴定;将溶磷木霉菌接种至辣椒幼苗根部后,采用常规试验方法测定溶磷木霉菌对辣椒生长性状、品质及根际土壤养分的影响,以期达到用溶磷木霉菌促进辣椒根际土中难溶性磷转化成速效性磷,减少磷肥施用量的目的。结果表明:溶磷木霉菌tang-10-11为拟康氏木霉(Trichoderma pseudokoningii),其溶磷透明圈直径为3.72 cm,显著高于其它木霉菌株。用100倍、300倍、500倍溶磷木霉菌tang-10-11处理后的辣椒根际速效磷含量分别为45.23、50.47、44.00 mg·kg^(-1),均显著高于清水对照。tang-10-11不仅可以提高辣椒产量,还可以提高辣椒维生素C含量和可溶性糖含量。因此,溶磷木霉菌tang-10-11可以促进辣椒根际土壤难溶性磷转化成速效性磷,从而促进辣椒植株生长,提高产量和品质。Phosphorus-dissolving Trichoderma strains were screened from insoluble inorganic phosphorus media containing Ca_(3)(PO_(4))_(2),which as a kind of phosphorus-dissolving material,and identified by morphological characteristics and ITS rDNA sequence.The phosphorus-dissolving ability of the strains was determined by the halo zone methods,and the growth-promoting ability on growth traits,quality and rhizosphere soil nutrients of pepper of the Trichoderma strains were analyzed with conventional method in order to promote the transformation of insoluble phosphorus into available phosphorus in pepper rhizosphere soil by Trichoderma phosphate solubilizing and reduce the application amount of phosphorus fertilizer.The results showed that the phosphorus dissolving fungiwas identified as Trichoderma pseudokoningii,and phosphorus transparent circle diameter was3.72 cm,which was significantly higher than others.Available P contents in the rhizosphere of pepper treated with 100 times,300 times and 500 times of tang^(-1)0-11 were 45.23 mg·kg^(-1),50.47 mg·kg^(-1) and 44.00 mg·kg^(-1),respectively,which were significantly higher than those of control.Moreover,tang^(-1)0-11 could not only increase the pepper yield but also increase the vitamin C content and soluble sugar content.It can be concluded that phosphate solubilizing Trichodermatang^(-1)0-11 could promote the transformation of insoluble P into available P in the pepper rhizosphere soil and promote pepper growth,increase pepper yield and quality.

关 键 词：溶磷木霉菌 拟康氏木霉 辣椒根际养分 促生作用 

分 类 号：S641.3[农业科学—蔬菜学]