加减苍附导痰汤治疗多囊卵巢综合征的网络药理学机制  

Network Pharmacological Mechanism of Modified Cangfu Daotan Decoction for Treatment of Polycystic Ovary Syndrome

作　　者：龙泳伶[1] 杨芳 张瑶 曾蕾[2] 卢如玲[2] 余璟玮 张如月 LONG Yong-Ling;YANG Fang;ZHANG Yao;ZENG Lei;LU Ru-Ling;YU Jing-Wei;ZHANG Ru-Yue(School of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;Panyu District Hexian Commemoration Hospital,Guangzhou 510000 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学第一附属医院,广东广州510405 [3]广州市番禺区何贤纪念医院,广东广州510000

出　　处：《广州中医药大学学报》2021年第7期1432-1439,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81804135);广东省自然科学基金资助项目(编号:2019A1515010777);罗元恺滋肾育胎丸中青年科研基金资助项目(编号:20190802)。

摘　　要：【目的】采用网络药理学方法探讨加减苍附导痰汤治疗多囊卵巢综合征的作用机制。【方法】使用中药系统药理学数据库与分析平台(TCMSP)筛选加减苍附导痰汤中中药的活性成分,通过UniProt数据库获得活性成分可能的作用靶点,应用GeneCards数据库获得多囊卵巢综合征的作用靶点,利用R数据包VennDiagram对加减苍附导痰汤潜在活性成分靶基因和多囊卵巢综合征相关的靶基因制作韦恩图,筛选交集基因,并将交集的基因构建蛋白相互作用网络,最后利用R数据包colorspace、stringi、DOSE、clusterProfiler、pathview对交集靶基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)信号通路分析,并用Omicshare在线制图软件将其结果可视化。【结果】筛选出加减苍附导痰汤活性成分159个,预测获得与多囊卵巢综合征相关靶点2270个,蛋白相互作用网络筛选出关键靶点89个。其主要与细胞炎症、增殖、分化、凋亡等生物学过程有关,可以作用于动脉粥样硬化信号通路、甲状腺激素信号通路、糖尿病并发症中的晚期糖基化终末产物-晚期糖基化终末产物受体(AGE-RAGE)信号通路、P53信号通路、内分泌拮抗信号通路、催乳素信号通路、雌激素信号通路中的关键靶点。【结论】加减苍附导痰汤治疗多囊卵巢综合征的作用机制与抑制炎症、凋亡、内分泌调节、组织稳态调节等途径有关。Objective To explore the mechanism of modified Cangfu Daotan Decoction for the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods The active ingredients of traditional Chinese medicines in modified Cangfu Daotan Decoction was screened out by Traditional Chinese Medicine Systems Pharmacology(TCMSP),and then the possible targets of the active ingredients were obtained by UniProt database,and the targets of PCOS were obtained by GeneCard database.A Venn diagram for PCOS-related target genes and modified Cangfu Daotan Decoction-related target genes was established by R data packet VennDiagram to achieve the intersection genes,and the obtained intersection genes were constructed a protein interaction relationship(PPI)network.Finally,the intersection target genes were given Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway analysis by R data package such as colorspace,stringi,DOSE,clusterProfiler,and pathview,and the results were visualized by Omicshare online mapping software.Results A total of 159 active ingredients in modified Cangfu Daotan Decoction were screened out,and 2270 PCOS-related target genes were obtained,ultimately,89 key targets were screened by protein interaction network.It plays an important roles in the biological processes such as cell inflammation,proliferation,differentiation,and apoptosis,etc.,and in the key targets of atherosclerosis signaling pathway,thyroid hormone signaling pathway,and advanced glycation end product(AGE)-receptor for AGEs(RAGE)signaling pathway,P53 signaling pathway,endocrine resistance signaling pathway,prolactin signaling pathway,and estrogen signaling pathway in diabetes mellitus complications.Conclusion The mechanism of modified Cangfu Daotan Decoction for the treatment of PCOS is related to inhibition of inflammation,apoptosis,endocrine regulation,steady-state tissue regulation,and other pathways.

关 键 词：加减苍附导痰汤 多囊卵巢综合征 网络药理学 炎症抑制 凋亡 内分泌调节 组织稳态调节 

分 类 号：R285.5[医药卫生—中药学]