肝藏血中肝郁血滞病机的实验探查  

Experimental Study on Pathogenesis of Liver Depression and Blood Stagnation in Liver Storing Blood

作　　者：钱梦[1] 岳晨[1] 杨宗纯 朱昊如 范方馨 谢鸣[1] QIAN Meng;YUE Chen;YANG Zong-Chun;ZHU Hao-Ru;FAN Fang-Xin;XIE Ming(Dept.of Science of Herbal Prescription,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学方剂学系,北京100029

出　　处：《广州中医药大学学报》2021年第7期1472-1480,共9页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81173193)。

摘　　要：【目的】探查肝藏血中肝郁血滞病机的现代内涵。【方法】将40只雄性大鼠随机分为正常组和模型组各20只。模型组采用慢性束缚应激法建立肝郁证模型,记录各组大鼠的不同时间点的外观行为与体质量的变化,于第2周末和第4周末进行糖水实验和旷场实验及检测下述指标水平:下丘脑去甲肾上腺素(NE);血浆5-羟色胺(5-HT)、皮质酮(CORT)、抗凝辅助因子抗凝血酶(AT-Ⅲ)、蛋白C(PC)及游离蛋白S(FPS);不同切变率下的全血黏度及血浆黏度,凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原(FIB),血小板聚集率。【结果】(1)肝郁相关指标。与正常组比较,模型组大鼠可见:体质量第1~4周均显著降低,活动状态和情绪睡眠积分均明显升高(P<0.05或P<0.01);糖水偏嗜度第2、4周均明显降低(P<0.05或P<0.01);爬行里程数第2周明显增加(P<0.01)、中央区停留时间明显降低(P<0.05),中央区停留时间、爬行里程数第4周均明显降低(P<0.05或P<0.01);血浆5-HT水平第2、4周均明显降低(P<0.05或P<0.01),血浆CORT水平第4周明显升高(P<0.05),下丘脑NE第4周水平明显降低(P<0.01)。(2)血滞相关指标。与正常组比较,模型组大鼠可见:第2周的PT、APTT、TT均明显降低(P<0.01),FIB明显升高(P<0.05),3 min血小板聚集率明显升高(P<0.01);第4周的中切、高切全血黏度和血浆黏度均明显升高(P<0.01),PT和APTT均明显降低(P<0.01),FIB明显升高(P<0.05),1、3、5 min血小板聚集率均明显升高(P<0.01),PC、FPS、AT-Ⅲ均明显降低(P<0.01)。【结论】慢性束缚应激肝郁证模型大鼠造模期间先后出现不同程度的肝郁证表现,而且还伴有血液黏度增高和凝血功能增强及肝脏对凝血因子及抗凝辅助因子平衡调节的异常。该结果在一定程度上可阐释中医肝郁血滞病机的现代内涵。Objective To explore the modern connotation on pathogenesis of liver and blood stagnation in liver storing blood.Methods Forty male rats were randomly divided into normal group and model group,20 rats in each group.The rats in the model group was induced into liver stagnation syndrome model by chronic restraint stress(CRS)method.The changes of appearance behavior and body mass of rats in each group were recorded regularly.Sugar water test and open field test were carried out at the second and the fourth weekend,and the indexed were determined as follows:hypothalamic norepinephrine(NE);plasma 5-hydroxytryptamine(5-HT),corticosterone(CORT),anticoagulant cofactors antithrombin(AT-Ⅲ),protein C(PC)and free protein S(FPS);whole blood viscosity and plasma viscosity under different shear rates,prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT)and fibrinogen(FIB),and platelet aggregation rate.Results(1)Liver qi depression related indicators.Compared with the normal group,the changes in the model group were as follows:the rat body mass was decreased significantly,active state and emotional sleep scores were significantly increased from the first to the fourth week(P<0.05 or P<0.01);the sugar water preference was significantly decreased in the second and the fourth week(P<0.05 or P<0.01);the crawling mileage was increased significantly(P<0.01)and central region residence time was decreased(P<0.05)in the second week,and the central region residence time and crawling mileage were decreased significantly in the fourth week(P<0.05 or P<0.01).The plasma level of 5-HT was significantly decreased in the second and the fourth weeks(P<0.05 or P<0.01),plasma level of CORT was increased significantly(P<0.05)and hypothalamic level of NE was decreased significantly(P<0.01)in the fourth week.(2)Blood stasis related indexes.Compared with the normal group,the changes in the model group were as follows:PT,APTT,and TT were significantly decreased(P<0.05 or P<0.01),FIB and the platelet aggregation rate in 3 m

关 键 词：肝郁证 肝郁血滞 神经内分泌 血液流变 凝血功能 动物模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]