一种水溶液中裸眼识别HSO_(3)^(-)/SO_(3)^(2-)的长波长荧光探针及其应用  

A Long-Wavelength Fluorescent Probe for Naked Eye Recognition of HSO_(3)^(-)/SO_(3)^(2-) in Aqueous Solution and Its Application

作　　者：王超[1] 王鑫[1] 钟克利[1] 侯淑华[1] 燕小梅[2] 边延江[1] 汤立军[1] Wang Chao;Wang Xin;Zhong Keli;Hou Shuhua;Yan Xiaomei;Bian Yanjiang;Tang Lijun(College of Chemistry and Material Engineering,Bohai University,Jinzhou,Liaoning 121013;College of Laboratory Medicine,Dalian Medical University,Dalian,Liaoning 116044)

机构地区：[1]渤海大学化学与材料工程学院,辽宁锦州121013 [2]大连医科大学检验医学院,辽宁大连116044

出　　处：《有机化学》2021年第6期2417-2423,共7页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.21878023,U1608222);辽宁特聘教授支持计划资助项目。

摘　　要：合成了一种(E)-2-(3-氰基-4-(3-甲酰基-4-羟基苯乙烯基)-5,5-二甲基呋喃-2(5H)-亚烷基)丙二腈衍生物CDM, ^(1)H NMR、^(13)C NMR和HRMS验证了其结构. CDM在MeCN/Tris (V∶V=2∶8, pH=7.4)溶液中通过荧光信号"ON-OFF"识别HSO_(3)^(-)/SO_(3)^(2-),颜色由粉色变为无色.探针CDM具有良好的抗干扰能力和较快的响应速度(200s),pH适用范围为7~14,荧光光谱和紫外吸收光谱的检测限分别为6.07和1.59μmol/L.实验研究表明,CDM能检测真实水样和糖样中的HSO_(3)^(-)/SO_(3)^(2-),并能够对活细胞中的HSO_(3)^(-)/SO_(3)^(2-)进行荧光成像.3-Cyano-4-(3-formyl-4-hydroxystyryl)-5,5-dimethylfuran-2(5H)-alkylene)malononitrile derivative CDM was synthesized. The correctness of its structure was verified by 1H NMR, 13C NMR and HRMS. CDM can recognize HSO_(3)^(-)/SO_(3)^(2-) with the naked eye through the fluorescent signal "ON-OFF" in MeCN/Tris(V∶V=2∶8, pH=7.4) solution, and the color changes from pink to colorless. The probe CDM has good anti-interference ability and fast response speed(200 s), the pH applicable range is 7~14, and the detection limits of the fluorescence and UV absorption spectrum are 6.07 and 1.59 μmol/L. The experimental research shows that CDM can detect HSO_(3)^(-)/SO_(3)^(2-) in real water samples and sugar samples and, can perform fluorescent image HSO_(3)^(-)/SO_(3)^(2-) in living cells.

关 键 词：荧光探针 HSO_(3)^(-)/SO_(3)^(2-) 长波长 裸眼识别 细胞成像 

分 类 号：O657.3[理学—分析化学]