T淋巴细胞亚群检测、锌剂治疗在病毒及细菌感染性腹泻中的应用  

Distribution of T lymphocyte subsets in viral and bacterial infectious diarrhea and analysis of zinc treatment

作　　者：许鑫松 朱焰[1] 路攀 XU Xin-song;ZHU Yan;LU Pan(Department of Pediatrics,Huzhou Normal College Affiliated Huzhou City First People's Hospital,Huzhou 313000,Zhejiang,China)

机构地区：[1]湖州市第一人民医院(湖州师范学院附属第一医院)儿科,浙江湖州313000

出　　处：《广东医学》2021年第7期792-795,共4页Guangdong Medical Journal

基　　金：浙江省医药卫生科技计划项目(2019KY209);湖州市科学技术局项目(2018GY18)。

摘　　要：目的观察外周血T淋巴细胞亚群在病毒及细菌感染性腹泻中的分布及锌剂在治疗中的疗效分析,探讨锌剂治疗与感染性腹泻及T淋巴细胞亚群分布之间联系。方法随机选取住院的轻中度感染性腹泻患儿138例,其中病毒感染75例,细菌感染63例,另随机选取健康儿童60例。分为病毒感染组(n=75)、细菌感染组(n=63)、健康对照组(n=60)。均采用流式细胞仪检测外周血T淋巴细胞亚群(CD4^(+)比例、CD8^(+)比例、CD4^(+)/CD8^(+)值),比较各组间变化及意义。其中病毒感染组中有33例予锌剂治疗,42例未予锌剂治疗,分为病毒补锌组(n=33)、病毒对照组(n=42);细菌感染组中有27例予锌剂治疗,36例未予锌剂治疗,分为细菌补锌组(n=27)、细菌对照组(n=36),分析锌剂治疗在病毒及细菌感染性腹泻中的疗效。病情恢复后随访病例中补锌组及对照组各随机抽取病例15例,比较T淋巴细胞亚群各数值的变化。结果病毒感染组及细菌感染组CD4^(+)比例、CD4^(+)/CD8^(+)值均低于健康对照组(P<0.05),CD8^(+)比例均高于健康对照组(P<0.05);病毒感染组与细菌感染组之间各数据差异无统计学意义(P>0.05)。各组中补锌组的疗效高于对照组。随访病例CD4^(+)比例、CD4^(+)/CD8^(+)值明显升高,CD8^(+)比例明显下降,且补锌组恢复更明显(P<0.05)。结论病毒及细菌感染性腹泻患儿外周血T淋巴细胞亚群值较正常患儿有明显变化,补锌在病毒及细菌感染性腹泻治疗中均有助于症状及T淋巴细胞亚群值的恢复。Objective To observe the distribution of T lymphocyte subsets in peripheral blood in viral and bacterial infectious diarrhea,and to analyze the therapeutic effect of zinc in treatment.To investigate the correlation among zinc treatment and infectious diarrhea and the distribution of T lymphocyte subsets in peripheral blood.Methods A total of 138 cases of mild to moderate infectious diarrhea children admitted to the department of pediatrics of the first affiliated hospital of Huzhou normal university from September 2018 to August 2019 were randomly selected,including 75 cases(viral infection group)of viral infection and 63 cases of bacterial infection(bacterial infection group).Another 60 healthy children were randomly selected as the control group.Flow cytometry was used to detect the peripheral blood T lymphocyte subsets(CD4^(+) ratio,CD8^(+) ratio,CD4^(+) /CD8^(+) value),and to compare the changes and significance of each group.Among them,33 cases in the virus infection group were treated with zinc,and 42 cases were not treated with zinc,which were divided into virus zinc supplementation group(n=33)and virus control group(n=42).In the bacterial infection group,27 cases were treated with zinc,and 36 cases were not treated with zinc.They were divided into the bacterial zinc supplementation group(n=27)and the bacterial control group(n=36).The efficacy of zinc in viral and bacterial infectious diarrhea was analyzed.After recovery,15 cases were randomly selected from zinc supplementation group and control group to compare the changes of T lymphocyte subsets.Results CD4^(+) ratio and CD4^(+) /CD8^(+) value in viral infection group and bacterial infection group were lower than those in control group(P<0.05),and CD8^(+) ratio was significantly higher than that in control group(P<0.05).There was no significant difference between virus group and bacteria group.The effect of zinc supplementation in each group was higher than that in the control group.The follow-up cases showed that the CD4^(+) ratio and CD4^(+) /CD8^(+) v

关 键 词：腹泻 T淋巴细胞亚群 锌 细胞免疫 

分 类 号：K446[历史地理—历史学] R453[历史地理—世界史]