4种不同中药单体对诺如病毒感染性肠炎小鼠细胞TNF-α、IL-1β、IL-6水平及PKR/p-PKR影响  

Effects of Four Different Chinese Medicine Monomers on the Levels of TNF-α,IL-1 β,IL-6 and PKR/p-PKR in Norovirus Infected Enteritis Mice

作　　者：华云玮 朱凌宇[2] 林俊儒[2] HUA Yunwei;ZHU Lingyu;LIN Junru(Shanghai Integrated Hospital of Traditional Chinese and Western Medicine,Shanghai 200082,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200003,China)

机构地区：[1]上海中医药大学附属上海市中西医结合医院,上海200082 [2]上海中医药大学附属龙华医院,上海200003

出　　处：《辽宁中医药大学学报》2021年第6期24-27,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：上海市自然科学基金(16ZR1433800)。

摘　　要：目的分析黄芪苷、绿原酸、儿茶素以及马钱酸4种不同中药单体对诺如病毒感染性肠炎小鼠细胞血清肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白介素1β(interleukin-1β,IL-1β)、白介素6(interleukin-6,IL-6)水平及双链核糖核酸依赖的蛋白激酶R(ribonucleic acid kinase repoter,PKR)/p-PKR的影响。方法该研究采用小鼠小肠黏膜上皮细胞(mice intestinal epithelial cells,MIECs)作为研究对象,小鼠诺如病毒感染小鼠小肠黏膜上皮细胞,Western blot方法分析4种不同中药单体对感染性肠炎模型PKR、p-PKR水平的影响;ELISA方法检测黄芪苷和儿茶素两种中药单体对感染性肠炎模型TNF-α、IL-1β、IL-6水平的影响。结果感染性肠炎模型的PKR、p-PKR随着时间的延长,其水平呈现显著升高趋势;其中黄芪苷组和儿茶素组抑制PKR的磷酸化(p-PKR)。通过对感染性肠炎模型TNF-α、IL-1β、IL-6水平的影响分析,发现黄芪苷组和儿茶素组显著抑制感染性肠炎模型的TNF-α、IL-1β和IL-6水平。结论黄芪苷和儿茶素相比其他两种中药单体,显著抑制PKR/p-PKR信号的转导,同时抑制TNF-α、IL-1β、IL-6的分泌,降低病灶的炎性反应。Objective To analyze the effects of chlorogenic acid four different monomers of traditional Chinese medicine on serum levels of tumor necrosis factor(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and double-stranded RNA-dependent ribonucleic acid kinase repoter(PKR)/p-PKR in mice with norovirus infectious enteritis. Methods Mouse intestinal epithelial cells(MIECs)were used as research objects in this study. Mouse norovirus infection was used to infect mouse intestinal epithelial cells to analyze PKR/p-PKR in the infectious enteritis model. The analysis of the influence of four different traditional Chinese medicine monomers on the levels of PKR and p-PKR in the infectious enteritis model was carried out. Effects of four different monomers on levels of TNF-α,IL-1β and IL-6 in infected enteritis model were analyzed. Results The levels of PKR and p-PKR in infective enteritis model increased significantly with the extension of time. Astragaloside and PKR/p-PKR signal transduction inhibited PKR phosphorylation. Though analysis of the levels of TNF-α,IL-1β and IL-6 in the model of infectious enteritis,we found that astragaloside group and catechin group significantly inhibited the levels of TNF-α,IL-1β and IL-6 in the model of infectious enteritis. Conclusion Compared with the other two traditional Chinese medicine monomers,astragaloside and catechin significantly inhibited PKR/p-PKR signal transduction and colateralization,meanwhile inhibited the secretion of TNF-α,IL-1β and IL-6,and reduced the inflammatory response of lesions.

关 键 词：感染性肠炎 黄芪苷 儿茶素 诺如病毒 PKR/p-PKR信号转导 

分 类 号：R516.1[医药卫生—内科学]