纳米金生物传感器的制备及其在汞离子检测中应用  

Preparation of gold nanoparticles biosensors and the application in mercury (Ⅱ) ions detection

作　　者：马静 金葆康[1] MA Jing;JIN Baokang(College of Chemistry and Chemical Engineering,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学化学化工学院,安徽合肥230601

出　　处：《安徽大学学报（自然科学版）》2021年第4期79-87,共9页Journal of Anhui University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(22076001)。

摘　　要：基于T-Hg^(2+)-T结构,在纳米金修饰电极(AuNPs/Au)表面构建了一种灵敏检测Hg^(2+)的电化学生物传感器.首先,在金(Au)表面电沉积AuNPs作为基底,增加ssDNA的引入量,50℃下硫堇(Th)依靠T和Hg^(2+)的特异性结合迅速嵌入ssDNA双螺旋内部,基于插入的电化学信号测定Hg^(2+).在最佳条件下,传感器对Hg^(2+)检测的线性范围为10~100 nmol·L^(-1),检出限低至7.4 nmol·L^(-1).传感器展现出良好的选择性.A sensitive electrochemical biosensor for detection of Hg^(2+)was prepared on the surface of AuNPs/Au based on the T-Hg^(2+)-T structure.Firstly,AuNPs were electrodeposited on the surface of Au,which increased the amount of ssDNA introduced.At 50℃,thionine(Th)was quickly embedded in the ssDNA double helices by the specific binding of T and Hg^(2+).Hg^(2+)was determined by the insertion of electrochemical signals.Under the optimal conditions,the linear range of the biosensor to Hg^(2+)was in the range of 10-100 nmol·L^(-1),and the detection limit was as low as 7.4 nmol·L^(-1).The sensor showed good selectivity.

关 键 词：生物传感器 纳米金 DNA Hg^(2+) 硫堇 

分 类 号：O646[理学—物理化学]