寻常型银屑病三种中医证型外周血单核细胞基因芯片与miRNA芯片关联分析  

Correlation Analysis of Gene Chip and miRNA Chip of Peripheral Blood Mononuclear Cells in Psoriasis Patients of Three Traditional Chinese Medicine Syndromes

作　　者：卢月 亓垚 陈曲波 韩凌 郜恒骏[2] 卢传坚 LU Yue;QI Yao;CHEN Qu-bo;HAN Ling;GAO Heng-jun;LU Chuan-jian(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510006,China;Shanghai Molecular Medicine Engineering Technology Research Center,Shanghai 201203,China;Guangdong Key Laboratory of Clinical Research on TCM Syndromes,Guangzhou Guangdong 510006,China)

机构地区：[1]广州中医药大学第二附属医院,广东广州510006 [2]生物芯片上海国家工程研究中心,上海201203 [3]广东省中医证候临床研究重点实验室,广东广州510006

出　　处：《中医药导报》2021年第6期142-145,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省科技计划项目(2019A1515010636,2020A1515010607,2020B1111100006);广东省教育厅项目(2018KQNCX046);广东省中医药科学院研究专项(YN2016XP02,YN2018RBA02,YN2019QJ04)。

摘　　要：目的:分析寻常型银屑病三种不同中医证型患者外周血单核细胞基因和mi RNA的表达,并进行关联分析。方法:收集寻常型银屑病三种中医证型(血瘀证、血燥证、血热证)患者62例和健康对照人群10人的外周血,检测外周血单核细胞的基因表达谱和mi RNA表达谱,利用DIANA在线工具及其数据库对基因芯片和mi RNA芯片结果进行关联分析。结果:与健康对照组比较,三种证型共有的差异mi RNA为hsa-miR-485-3p,三种证型共有的差异基因为RBMS1和FAM98B,血瘀证存在靶标关系的特有差异mi RNA和基因为159对,血燥证存在靶标关系的特有差异mi RNA和基因为15对,血热证不存在证型特有差异mi RNA。结论:差异mi RNA通过调控其靶基因的表达参与银屑病的发生及中医分型。Objective:To analyze the expression of genes and miRNA in peripheral blood mononuclear cells in psoriasis vulgaris patients with three different TCM syndromes,and to conduct correlation analysis.Methods:Peripheral blood was collected from 62 patients with psoriasis vulgaris of the three TCM syndromes(blood stasis syndrome,blood dryness syndrome and blood heat syndrome)and 10 healthy controls,and then the gene expression profile and miRNA expression profile of peripheral blood mononuclear cells were detected.DIANA online tools and databases were applied to correlate the results of gene chip and miRNA chip.Results:Comparing with the healthy control group,the differential miRNA shared by the three syndromes was hsa-miR-485-3 p,and the differential genes shared by the three syndromes were RBMS1 and FAM98 B.There are 159 pairs of miRNAs and genes with a specific target relationship in blood stasis syndrome,and 15 pairs of miRNAs and genes with a target relationship in blood dryness syndrome.There was no miRNAs and genes with a specific target relationship in blood heat syndrome.Conclusion:The differential miRNAs participate in the occurrence of psoriasis and TCM syndromes by regulating the expression of their target genes.

关 键 词：银屑病 中医证型 基因芯片 MIRNA芯片 外周血单核细胞 关联分析 

分 类 号：R275.9[医药卫生—中西医结合]