葛根对高脂喂养去卵巢小鼠异常代谢表型的作用  

Effect of Radix Puerariae on Aberrant Metabolic Phenotype in Ovariectomized Mice Fed by HighfatDiet

作　　者：刘素嬛 上官兆水[2] 排孜丽亚·买提阿西木[2] 陈启亮 李灿东 LIU Su-huan;SHANGGUAN Zhao-shui;MAITIAXIMU Pai-zi-li-ya;CHEN Qi-liang;LI Can-dong(Research Base of Chinese Medicine Syndrome,Fujian University of Traditional Chinese Medicine,Fujian Key Laboratory of TCM Health State,LI Can-dong Qihuang Scholar Studio,Fuzhou,50122;Research Center for Translational Medicine,First Affiliated Hospital of Xiamen University,Fujian,361004)

机构地区：[1]福建中医药大学中医证研究基地,福建省中医健康状态辨识重点实验室,李灿东岐黄学者工作室,福州350122 [2]厦门大学附属第一医院转化医学研究中心,福建361004

出　　处：《中国中西医结合杂志》2021年第7期830-835,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81270901)。

摘　　要：目的探讨葛根(RP)是否对高脂饲料(HFD)诱导的卵巢切除(OVX)小鼠代谢表型异常发挥雌激素样保护作用。方法将30只雌性小鼠随机分为正常饮食假手术(ND)组、高脂饲料假手术(HFD)组、高脂饲料卵巢切除(HFD+OVX)组、高脂饲料卵巢切除合并雌激素治疗(HFD+OVX+E2)组、高脂饲料卵巢切除合并低剂量葛根(0.4 g/kg)治疗(HFD+OVX+RP 0.4)组、高脂饲料卵巢切除合并高剂量葛根(0.8 g/kg)治疗(HFD+OVX+RP 0.8)组,每组5只。ND、HFD组小鼠行假手术,其余小鼠行OVX。高脂饲料喂养4周后开始给予OVX小鼠RP或E2治疗,观察其对小鼠代谢表型的作用,包括:体重、子宫重量、血糖、葡萄糖及胰岛素耐量、体成分分析、能量代谢等指标。结果与ND组比较,HFD组小鼠脂肪含量明显增加(P<0.05);与HFD组比较,HFD+OVX组瘦肉含量下降(P<0.05);血糖、葡萄糖耐量上升(P<0.05)。与HFD+OVX组比较,HFD+OVX+E2组小鼠体重和脂肪含量降低(P<0.01),子宫重量、瘦肉和体液含量增加(P<0.01),血糖、葡萄糖耐量和胰岛素耐量改善(P<0.05,P<0.01);HFD+OVX+RP 0.4、0.8组耗氧量、二氧化碳产量、产热量有降低的趋势,而自主活动量有升高的趋势,对小鼠体重、子宫重量、体成分含量、血糖水平无明显作用,差异均无统计学意义(P>0.05)。结论RP对高脂饲料诱导的OVX小鼠异常代谢表型无雌激素样保护作用。Objective To observe whether Radix Puerariae(RP)exerts estrogen-like protection on high-fat diet induced aberrant metabolic phenotype in ovariectomized mice.Methods Totally 30 female mice were randomly divided into 6 groups:normal diet with sham operation(ND),high-fat diet with sham operation(HFD),HFD with ovariectomy(HFD+OVX),HFD with ovariectomy and estrogen treatment(HFD+OVX+E2),HFD with ovariectomy and low-dose RP(0.4 g/kg)treatment(HFD+OVX+RP 0.4),and HFD with ovariectomy and highdose RP(0.8 g/kg)treatment(HFD+OVX+RP 0.8),5 mice in each group.ND and HFD mice received sham operation,and all the other mice received OVX.After four weeks HFD feeding,the ovariectomized mice started to receive RP at low and high doses or E2 treatment.Body weight,blood glucose,glucose tolerance,insulin tolerance,body composition,and energy metabolism were measured.Results Compared with that of the ND mice,the fat mass of the HFD mice significantly increased(P<0.05).Compared with the HFD group,lean meat content decreased,blood glucose and glucose tolerance significantly increased(P<0.05)in the HFD+OVX group(P<0.05).Compared with the HFD+OVX group,E2 treated mice had reduced body weight and fat mass(P<0.01),significantly increased uterine weight,lean mass and body fluid content(P<0.01),improved blood glucose,glucose tolerance,and insulin tolerance(P<0.05,P<0.01).RP at both 0.4 or 0.8 g/kg dose tended to reduce oxygen consumption,carbon dioxide production,caloric production,and elevate the amount of physical activity.RP had no effect on body weight,uterine weight,body composition,or blood glucose levels(P>0.05).Conclusion RP had no estrogen-like protection on abnormal metabolic phenotypes induced by high-fat feeding in ovariectomized mice.

关 键 词：葛根 雌激素 卵巢切除 代谢表型 能量稳态 

分 类 号：R285.5[医药卫生—中药学]