祛风宣肺方对咳嗽敏感性增高豚鼠TRPV1通道相关蛋白的影响  

Effect of Qufeng Xuanfei Recipe on TRPV1 Channel-associated Proteins in Guinea Pigs with Cough Hypersensitivity

作　　者：马建岭[1] 王颖[1,2] 史利卿 温绍惠[1] 李扭扭[1] 王丽云[1] 季坤[1] 董尚娟[1] 罗敬月 MA Jian-ling;WANG Ying;SHI Li-qing;WEN Shao-hui;LI Niu-niu;WANG Li-yun;JI Kun;DONG Shang-juan;LUO Jing-yue(Department of Respiration,Dong fang Hospitaly Beijing University of Chinese Medicine,Beijing,100078;Department of Respirationy Zhejiang Provincial Hospital of Traditional Chinese Medicine,Hangzhou,310006;Department of Traditional Chinese Medicine,Beijing Chest Hospital,Capital Medical University,Beijing,101149)

机构地区：[1]北京中医药大学东方医院呼吸热病科,北京100078 [2]浙江省中医院呼吸科,杭州310006 [3]首都医科大学附属北京胸科医院中医科,北京101149

出　　处：《中国中西医结合杂志》2021年第7期842-847,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：北京市自然科学基金资助项目(No.7184224,No.7192115);国家自然科学基金资助项目(No.81373588);北京中医药大学东方医院“1166”人才培养项目(No.040204001001002012);北京中医药大学科研基金资助项目(No.2017-JYB-JS-102)。

摘　　要：目的探讨祛风宣肺方对咳嗽敏感性增高豚鼠瞬时受体电位香草酸亚型1(TRPV1)通道相关蛋白的作用,以期探讨其疗效机制。方法48只SPF级豚鼠分为正常组(给予生理盐水)、模型组、西药组、中药组,每组12只,除正常组外,其余3组采用卵蛋白致敏的方法建立咳嗽敏感性增高豚鼠模型,模型组给予生理盐水,西药组给予辣椒平,中药组给予祛风宣肺方,干预1周后采用ELISA法测定血清中IL-6、IL-20水平、支气管肺泡灌洗液(BALF)中TRPV1、P物质(SP)、降钙素基因相关肽(CGRP)蛋白水平,Western Blot法测定肺组织中TRPV1、神经激肽-1受体(NK-1R)、CGRP蛋白表达。结果与正常组比较,模型组血清IL-6、IL-20、BALF中TRPV1、SP、CGRP及肺组织中TRPV1、NK-1R、CGRP水平升高(P<0.01);与模型组比较,西药组及中药组血清IL-6、IL-20、BALF中TRPV1、SP、CGRP水平降低(P<0.01),但中药组SP水平与模型组比较,差异无统计学意义(P>0.05);与西药组比较,中药组BALF中TRPV1、SP、CGRP水平升高(P<0.05,P<0.01)。与模型组比较,西药组、中药组肺组织中TRPV1、NK-1R、CGRP蛋白表达下降(P<0.05,P<0.01);与西药组比较,中药组肺组织中TRPV1、NK-1R、CGRP表达升高(P<0.01)。结论抑制TRPV1通道进而降低气道神经源性炎症可能是祛风宣肺方的重要疗效机制之一。Objective To study the effect of Qufeng Xuanfei Recipe(QFXFR)on transient receptor potential vanilloid 1(TRPV1)channel-associated proteins in guinea pigs with cough hypersensitivity,so as to study its therapeutic mechanism.Methods Totally 48 SPF grade guinea pigs were randomly divided into normal group,model group,Western medicine group(WM),traditional Chinese medicine group(TCM),12 in each group.The guinea pigs model of cough hypersensitivity was established by ovalbumin sensitization in all guinea pigs except those in the normal group.Normal saline was administered to those in normal group.Capsazepine was administered to those in the WM group.QFXFR was administered to those in the TCM group.After one week of drug intervention,the serum levels of IL-6 and IL-20,the levels of TRPV1,substance P(SP),and calcitonin gene-related peptide(CGRP)protein in bronchoalveolar lavage fluid(BALF)were measured by ELISA.The levels of TRPV1,NK-1 R,and CGRP protein expressions in lung tissue were measured by Western Blot.Results Compared with the normal group,the serum levels IL-6 and IL-20,levels of TRPV1,SP,and CGRP in BALF,levels of TRPV1,NK-1 R,and CGRP in lung tissue significantly increased in the model group(P<0.01).Compared with the model group,the serum levels of IL-6 and IL-20,levels of TRPV1,SP,and CGRP in BALF significantly decreased in WM group and TCM group(P<0.01).But there was no significant difference in SP level between the WM group and TCM group(P>0.05).Compared with the WM group,the levels of TRPV1,SP,and CGRP in BALF significantly increased in the TCM group(P<0.05,P<0.01).Compared with the model group,the levels of TRPV1,NK-1 R,and CGRP in lung tissue significantly increased in the WM group and the TCM group(P<0.05,P<0.01).Compared with the WM group,the levels of TRPV1,NK-1 R,and CGRP in lung tissue significantly increased in the TCM group(P<0.01).Conclusion Inhibiting TRPV1 channel and further reducing airway neurogenic inflammation might be one of important therapeutic mechanisms for QFXFR.

关 键 词：慢性咳嗽 祛风宣肺方 咳嗽敏感性增高 气道神经源性炎症 瞬时受体电位香草酸亚型1 

分 类 号：R285.5[医药卫生—中药学]