芸薹属物种(B.napus,B.rapa,B.oleracea)PHB基因的生物信息学分析  

Bioinformatics Analysis of PHB Gene in Brassica Species(B.napus,B.rapa,B.oleracea)

作　　者：王晗 闫敏 许晔 王仁雷 刘少华 Wang Han;Yan Min;Xu Ye;Wang Renlei;Liu Shaohua(Jiangsu Key Laboratory for Biofunctional Molecules,College of Life Science and Chemistry,Jiangsu Second Normal University,Nanjing,211200)

机构地区：[1]江苏第二师范学院生命科学与化学化工学院,江苏省生物功能分子重点建设实验室,南京211200

出　　处：《分子植物育种》2021年第13期4232-4241,共10页Molecular Plant Breeding

基　　金：江苏省自然科学基金资助项目(BK20170756);国家自然科学基金项目(31700280);江苏省高等学校自然科学基金项目(17KJB180002)共同资助。

摘　　要：HD-ZIPⅢ基因家族参与植物胚胎发生、分生组织形成、叶极性建立和维管组织分化等过程。本研究利用生物信息学方法,对HD-ZIPⅢ成员之一PHABULOSA(PHB)进行鉴定,在甘蓝型油菜、白菜和甘蓝中共获得7个PHB成员,并对其核苷酸和氨基酸序列的基本特征、结构域、保守基序、基因结构和系统进化关系进行了分析。结果表明,这些PHB蛋白编码不稳定的亲水性蛋白,所有成员均含有Homeobox、bZIP、START和MEKHLA保守结构域,PHB位点在三个物种间的进化符合禹氏三角理论。序列分析及测序结果显示白菜的两个PHB基因在miR165、miR166结合位点处存在一个碱基差异。本研究为进一步揭示芸薹属物种PHB基因功能提供了依据。ClassⅢhomeodomain leucine zipper(HD-ZIPⅢ)family genes are involved in the processes of embryogenesis,apical meristem formation,the establishment of leaf polarity and vascular differentiation.This study uses bioinformatics methods to identify one of the HD-ZIPⅢmembers,PHABULOSA(PHB).A total of seven PHB genes were identified from B.napus,B.rapa and B.oleracea.The protein characteristics,conserved domains,gene structure and evolutionary relationship were analyzed.The results showed that these PHB proteins encoded unstable and hydrophilic proteins and all members contained Homeobox,bZIP,START and MEKHLA domains.Phylogenetic analysis indicated the evolution of PHB sites in the three species was consistent with U’triangle theory.Sequence analysis and sequencing results showed that the two PHB genes of Brassica rapa exhibited a different base at the miR165,miR166 binding sites.This study provides a basis for further research on PHB gene in Brassica.

关 键 词：芸薹属 PHB miR165 miR166 生物信息学 

分 类 号：Q943.2[生物学—植物学]