非生物胁迫下海马齿SpSOS1基因的表达及响应ABA模式分析  

Expression Pattern Analysis of SpSOS1 from Sesuvium portulacastrum under Abiotic Stresses and the Response to ABA

作　　者：张婷婷 康宇乾 李雨欣 王健[2] 宋希强[2] 周扬[1,3] Zhang Tingting;Kang Yuqian;Li Yuxin;Wang Jian;Song Xiqiang;Zhou Yang(Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province,Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province,College of Horticulture,Hainan University,Haikou,570228;Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants,College of Forestry,Hainan University,Haikou,570228;Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海南省热带园艺作物品质调控重点实验室,海南省热带特色花木资源生物学重点实验室,海口570228 [2]海南大学林学院,教育部热带特色林木花卉遗传与种质创新重点实验室,海口570228 [3]海南大学,海南省耐盐作物生物技术重点实验室,海口570228

出　　处：《分子植物育种》2021年第13期4371-4377,共7页Molecular Plant Breeding

基　　金：海南省自然科学基金(318QN189);海南大学科研启动项目(KYQD(ZR)1845);海南省自然科学基金创新团队项目(2018CXTD331)共同资助。

摘　　要：为分析盐生植物海马齿(Sesuvium portulacastrum L.)SpSOS1基因的表达模式,本研究利用实时荧光定量PCR技术分析SpSOS1在海马齿不同组织以及不同胁迫条件下的表达情况。空间表达模式分析表明,SpSOS1在海马齿根中表达量最高,在花中的表达量最低。不同胁迫条件下SpSOS1基因的表达分析表明,在干旱、高温(42℃)、低温(4℃)以及氧化胁迫下表达量无明显变化;在ABA处理后,SpSOS1基因呈现上调表达,且在6 h表达量达到峰值,与盐胁迫处理下的表达趋势基本一致。为进一步探究盐胁迫下SpSOS1的上调表达是否受ABA的诱导,使用钨酸钠和氟啶酮作为ABA生物合成抑制剂和清除剂处理海马齿,结果表明,用钨酸钠和氟啶酮处理后,盐胁迫下SpSOS1的表达增加量从9.8倍分别降为起始表达量的1.8和2.1倍,表明盐胁迫下SpSOS1的上调受ABA信号途径诱导。本研究为进一步探究SpSOS1的耐盐调控途径提供参考依据。In order to analyze the expression pattern of the SpSOS1 gene in the halophyte Sesuvium portulacastrum,q RT-PCR was used to analyze the expressions in different tissues of S.portulacastrum and under different stresses.Spatial expression pattern analysis showed that the expression of SpSOS1 was highest in roots and lowest in flowers.The expression level of SpSOS1 gene has no significantly change under drought,high temperature(42℃),low temperature(4℃)and oxidative stress.However,the expression of SpSOS1 was induced by ABA treatment and reached to the highest level at 6 h after treatment,which was basically consistent with NaCl treatment.To explore whether the up-regulation of SpSOS1 upon salinity involves ABA signaling,tungstate and fluridone were chosen as the inhibitor and scavenger of ABA biosynthesis in plants of S.portulacastrum.The results showed that the expression increase of SpSOS1 under salt stress decreased from 9.8 times to 1.8 times and 2.1 times of the initial expression after treatment with tungstate and fludiazone,respectively,indicated that the up-regulation of SpSOS1 under salt stress was involved in ABA signaling pathway.This study provides a reference basis for further exploration of the salt tolerance regulation of SpSOS1.

关 键 词：海马齿(Sesuvium portulacastrum) SOS1 盐胁迫 ABA 基因表达 

分 类 号：Q943.2[生物学—植物学]