冬瓜SSR多态性标记遗传及杂种种子纯度鉴定  

Genetics of SSR Polymorphism Marker and Purity Identification of Hybrid Seeds in Wax Gourd(Benincasa hispida)

作　　者：陈婕英 刘政国[1] 汪春玲 陈庆明 程志魁 马连莲 苟纪权 Chen Jieying;Liu Zhengguo;Wang Chunling;Chen Qingming;Cheng Zhikui;Ma Lianlian;Gou Jiquan(Agricultural College,Guangxi University,Nanning,530001)

机构地区：[1]广西大学农学院,南宁530001

出　　处：《分子植物育种》2021年第13期4423-4428,共6页Molecular Plant Breeding

基　　金：广西科技重大专项(桂科AA17204026)资助。

摘　　要：种子纯度鉴定是种子应用于生产前的必经过程,是确保种子质量的关键,开发出一套准确有效、低廉便捷的冬瓜种子纯度鉴定方法能为种子质量提供技术支撑。本研究以‘金圆2号’、‘金圆3号’和‘绿仙子2号’3个冬瓜杂种为试验材料,筛选出2对具有共显性的SSR引物XD-18和XD-99用于冬瓜种子纯度鉴定。首先利用冬瓜杂种的F_2代分析这2个标记是否符合孟德尔式分离,若符合孟德尔式分离则再利用这2对引物分别对冬瓜杂种种子进行SSR分子标记纯度鉴定,并与田间种植纯度鉴定进行比较实验,结果显示:这2个标记在F2代群体中均符合1∶2∶1的孟德尔式分离,冬瓜杂种种子SSR分子标记纯度鉴定结果与田间种植纯度鉴定结果高度一致。本研究可为冬瓜乃至作物杂种种子的纯度鉴定提供一套更准确的技术体系。Seed purity identification is an indispensable process before seeds are used in production and is the key to ensuring seed quality.Developing an accurate,effective,inexpensive and convenient method for wax gourd seeds purity identification can provide technical support for seed quality.The co-dominant primers of XD-18 and XD-99 were chosen to test the seed purity of’Jinyuan2’,’Jinyuan3’and’Lüxianzi2’three wax gourd hybrids in this research.The F2 generations of wax gourd hybrids were used to analyze whether two SSR markers were in accordance with Mendelian separation law.If it is in accordance with Mendelian separation,then two pairs of primers were used to carry out SSR molecular marker purity identification of hybrid seeds of wax gourd,and a comparison experiment was performed between the SSR molecular marker purity identification and the field planting purity identification.The results showed that the 2 SSR markers were in accordance with the Mendelian separation law of 1∶2∶1 in the F2 generation population and SSR molecular marker purity identification of wax gourd hybrid seeds was highly consistent with the field planting purity identification.This study provides a more accurate technical system for the purity identification of wax gourd seeds and other crop hybrid seeds.

关 键 词：冬瓜 SSR标记 孟德尔式分离 杂种纯度 

分 类 号：S642.3[农业科学—蔬菜学]