基于Cytb及AFLP标记探讨仿石鲈科及相关科属分子系统进化关系  

Molecular phylogenetic relationships of Haemulidae species and related families/genera based on Cyt b and AFLP markers

作　　者：陈铭 范蔓桦 张癸新 李子博 李清清 李江涛 周萌 梁日深 林蠡 CHEN Ming;FAN Manhua;ZHANG Guixin;LI Zibo;LI Qingqing;LI Jiangtao;ZHOU Meng;LIANG Rishen;LIN Li(College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center,Guangzhou 510225,China;Guangdong Province Key Laboratory of Waterfowl Healthy Breeding,Guangzhou 510225,China;Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省水环境与水产品安全工程技术研究中心,广东广州510225 [3]广东省水禽健康养殖重点实验室,广东广州510225 [4]广州市水产病害与水禽养殖重点实验室,广东广州510225

出　　处：《中国水产科学》2021年第7期832-844,共13页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(31802300);广东省自然科学基金项目(2018A0303130104);广东省科技计划项目(2017A040403060);广东省重点领域研发计划项目(2019B020215001)。

摘　　要：为从分子水平澄清我国仿石鲈科(Haemulidae)鱼类分类争议,研究测定分析了仿石鲈科及其相关科属共66种鱼类的线粒体DNA细胞色素b(Cyt b)部分序列,结合AFLP标记信息,共同探讨仿石鲈科及相关争议科属系统分类关系。结果表明,在Cytb基因上,66种鱼类一致序列为729 bp,编码243个氨基酸;AFLP图谱中,6对引物组合共得到669条清晰条带,多态性条带669条,多态性比率100%。基于Cyt b基因利用最大似然法与AFLP标记基于UPGMA聚类法构建的系统进化树大致相同,进化树上,(1)仿石鲈科鱼类主要形成石鲈亚科(Haemulinae)与胡椒鲷亚科(Plectorhynchinae)两大分支,石鲈属(Pomadasys)、仿石鲈属(Haemulon)、异孔石鲈属(Anisotremus)等7个属聚为石鲈亚科分支,胡椒鲷属(Plectorhinchus)、少棘胡椒鲷属(Diagramma)与矶鲈属(Paraprstipoma)3个属聚为胡椒鲷亚科分支。(2)髭鲷属(Hapalogenys)与眶棘鲈属(Scolopsis)没有与仿石鲈科聚在一起,髭鲷属独自形成一支,眶棘鲈属与金线鱼科(Nemipteridae)聚为一支。(3)鯻科(Teraponidae)的种类也没有与仿石鲈科鱼类相聚,鯻科与舵鱼科(Kyphosidae)、石鲷科(Oplegnathidae)存在更近的亲缘关系。对于仿石鲈科及相关科属的分类争议,研究支持仿石鲈科分为石鲈亚科与胡椒鲷亚科两个亚科,以及鯻科、眶棘鲈属、髭鲷属不应该归为仿石鲈科的观点。Haemulidae fish,belonging to the suborder Percoidei of the order Perciformes,are important commercial marine fish species in China.Morphologically,classification controversies still exist among the genera and species of this family.To clarify such controversies,in this study,partial mitochondrial cytochrome b sequences of 66 Haemulidae species and their related families/genera were determined and analyzed.Combining this with AFLP marker information,the molecular phylogenetic relationships of Haemulidae and related species were then constructed and investigated.The phylogenetic trees were constructed based on the Cyt b gene sequences using the maximum likelihood method and based on AFLP markers using the UPGMA method.The results showed that in the Cyt b gene,739 bp homologous sequences from 66 species were obtained,with 243 amino acids encoded;in the AFLP fingerprints,based on six primer pairs,a total of 669 clear polymorphic bands were obtained,with a polymorphic ratio of 100%.Genetic distances among Haemulidae and related families/genera(Terapontidae,Scolopsis,Hapalogenys)calculated by MEGA 7.0 revealed that the distance values between Haemulidae and Terapontidae(Cyt b:0.191–0.275;AFLP:1.268–1.683),Haemulidae and Hapalogenys(Cyt b:0.180–0.297;AFLP:1.225–1.556),and Haemulidae and Scolopsis(Cyt b:0.208–0.290;AFLP:1.097–1.553)were all larger than the Haemulidae intra-genera genetic distance values(Cyt b:0.024–0.243;AFLP:0.690–1.362),indicating their distant relationship with Haemulidae.The phylogenetic trees constructed by Cyt b genes and AFLP marker information revealed similar topologies.In the phylogenetic tree the following was found:1)Haemulidae species were divided into two major groups,seven genera(including Pomadasys,Haemulon,and Anisotremus)were clustered into the Haemulinae group and three genera(namely,Plectorhinchus,Diagramma,and Parapristipoma)were clustered into the Plectorhynchinae group;2)the genera Hapalogenys and Scolopsis were not clustered together with the Haemulidae group.Hapal

关 键 词：仿石鲈科 系统分类 AFLP 细胞色素B 

分 类 号：S917[农业科学—水产科学] Q953[生物学—动物学]