Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system  

作　　者：Nanqi REN Han KANG Xiuheng WANG Nan LI 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China

出　　处：《Frontiers of Environmental Science & Engineering》2011年第2期277-282,共6页环境科学与工程前沿（英文）

基　　金：This research was supported by the National Nature Science Foundation of China(Grant No.50821002,National Creative Research Groups);Mega-Projects of Science Research for Water(No.2008ZX07207-005-003);the National Natural Science Foundation of China(Grant No.50638020).

摘　　要：Sequencing batch reactor(SBR)for enhanced biological phosphorus removal(EBPR)processes was used to investigate the impact of the temperature shock on the competition between phosphorus-accumulating organisms(PAOs)and glycogen accumulating organisms(GAOs)in start-up stage.During the 34 days operation,SBR was set with temperature variation(0-5 d,22±1℃;6-13 d,29±1℃;14-34 d,14±1℃).PAOs and GAOs were analyzed by fluorescent in situ hybridization(FISH),and intracellular polyphosphate granules were stained by Neisser-stain.The results showed that the influence of temperature shock on PAOs’abundance was more serious than that on GAOs in the enriching process.Under sudden and substantially temperature variation,from 22±1℃ to 29±1℃ and then to 14±1℃,the domination of PAOs was deteriorated.After temperature shock,PAOs’competitive advantages at low temperature that concluded in other study did not appear in our study.As mesophilic,GAOs(indicated by Alphaproteobacteria and Gammaproteobacteria)were more temperature adaptive and better grew and took the domination at 14±1℃ in the end.In the competition process,organisms of tetrad forming organisms(TFOs)-like shape which were considered as typical GAOs,were observed.With the evidence of poly-P granules containing by Neisser-straining and result of FISH,these organisms of TFOs-like shape were better to be assumed as adaption state or a special self-protecting shape of PAOs.

关 键 词：fluorescent in situ hybridization(FISH) tetrad forming organisms(TFOs) temperature variation enhanced biological phosphorus removal(EBPR) 

分 类 号：X70[环境科学与工程—环境工程]