马铃薯地方主栽品种“春薯4号”遗传转化体系的建立  

Establishment of Genetic Transformation System of a Main Local Potato Cultivar Chunshu 4

作　　者：李传龙 李楠[2] 朱旭 郝东云[2] 贺红霞[2] LI Chuanlong;LI Nan;ZHU Xu;HAO Dongyun;HE Hongxia(Academy of Life Sciences,Jilin Normal University,Siping 136000,China;Jilin Provincial Key Laboratory of Agricultural Biotechnology,Institute of Agriculture Bio-technology Research,Jilin Acad-emy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]吉林师范大学生命科学学院,四平136000 [2]吉林省农业生物技术重点实验室,吉林省农业科学院农业生物技术研究所,长春130033

出　　处：《吉林农业大学学报》2021年第3期289-296,共8页Journal of Jilin Agricultural University

基　　金：吉林省农业科技创新工程自由创新项目(CXGC2018ZY034,CXGC2018JC002)。

摘　　要：以吉林省马铃薯主栽品种"春薯4号"(Solanum tuberosum cv. Chunshu 4)无菌试管苗为材料,以茎段为外植体,采用L9(33)正交试验设计,研究植物激素对愈伤组织诱导和分化的影响;通过对遗传转化体系中的筛选剂浓度、抗生素种类及浓度等影响因素的优化,初步建立了马铃薯"春薯4号"的遗传转化体系。结果表明:最适合愈伤组织诱导的培养基配方为MS+2.0 mg/L 6苄氨基嘌呤(6-BA)+0.2 mg/L萘乙酸(NAA)+0.3 mg/L 2,4-二氯苯酚代乙酚(2,4-D),"春薯4号"茎段的愈伤组织诱导率最高,为98.57%,愈伤组织为淡绿色,呈致密的哑铃形;最适合愈伤组织分化的培养基为MS+3.0 mg/L 6-BA+1.5 mg/L吲哚乙酸(IAA)+1.0 mg/L玉米素(ZT),愈伤组织的分化出苗率最高,为54.1%,幼苗粗壮,畸形苗少;在遗传转化体系中,筛选剂双丙氨膦浓度为6.0 mg/L时,为愈伤组织最适筛选压,为4.5 mg/L时,为再生苗最适筛选压;农杆菌抑菌剂最适组合为500 mg/L头孢霉素+200 mg/L羧苄青霉素。Using sterile test-tube seedlings of main potato cultivar Chunshu 4(Solanum tuberosum cv. Chunshu 4) in Jilin province as materials and stem segments as explants, the effects of phytohormones on callus induction and differentiation were studied with L9(33) orthogonal design. The genetic transformation system of potato Chunshu 4 was preliminarily established by optimizing factors such as selection concentration, and type and concentration of antibiotic. The results showed that the most suitable callus induction medium was MS + 2.0 mg/L 6-benzidine(6-BA) + 0.2 mg/L naphthaleneacetic acid(NAA) + 0.3 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D), with which the callus induction rate of stem segment of Chunshu 4 was the highest(98.57%), and the callus was light green and dense dumbbell-shaped. The most suitable differentiation medium was MS + 3.0 mg/L 6-BA+1.5 mg/L indole-3-acetic acid(IAA) + 1.0 mg/L zeatin(ZT), with which the callus differentiation and seedling emergence rate was the highest(54.1%), and the seedlings were strong and the deformed seedlings were few. In the genetic transformation system, the optimum screening pressure for callus was bialaphos concentration of 6.0 mg/L, and the optimum screening pressure for regenerated seedlings was 4.5 mg/L. The optimum combination of Agrobacterium bacteriostatic agents was 500 mg/L cephalosporin + 200 mg/L carboxybenzylpenicillin.

关 键 词：马铃薯 农杆菌介导法 遗传转化体系 正交试验 植物激素 

分 类 号：S532[农业科学—作物学]