菜心BcSVP基因mRNA在异源嫁接体中的运输分析  

Analysis of mRNA transport of BcSVP of Chinese flowering cabbage in heterograft

作　　者：陶鹏[1] 赵彦婷[1] 岳智臣[1] 雷娟利[1] 李必元[1] TAO Peng;ZHAO Yanting;YUE Zhichen;LEI Juanli;LI Biyuan(Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2021年第7期1192-1198,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金(LY21C150006);浙江省农业新品种选育重大科技专项(2016C02051-6-2);国家自然科学基金(31601746)。

摘　　要：为研究菜心BcSVP基因mRNA的长距离运输,本研究将结球甘蓝茎尖作为接穗嫁接到砧木菜心的花序轴上,构建结球甘蓝/菜心异源嫁接体。基于菜心和结球甘蓝的转录组测序的read文库,拼接获得了菜心的BcSVP和结球甘蓝BoSVP基因编码序列和3’UTR序列。利用菜心BcSVP在3’UTR的种间差异序列(CF和CR),分别在异源嫁接(结球甘蓝/菜心嫁接)的接穗结球甘蓝茎尖的转录组测序read文库T01和T02中筛选到2条和1条来自砧木菜心的BcSVP的read。利用结球甘蓝BoSVP在3’UTR的种间差异序列(GF和GR),计算了结球甘蓝内源BoSVP基因的转录表达量,显示异源嫁接和同源嫁接的接穗结球甘蓝茎尖中BoSVP基因的表达量差异不明显。本研究鉴定了菜心BcSVP基因的mRNA在异源嫁接体中的长距离运输,对进一步研究菜心BcSVP基因的mRNA长距离运输机制及其生物学功能奠定了基础。For confirming mRNA long-transport of BcSVP gene,shoot apex of head cabbage was grafted on inflorescence stem of Chinese flowering cabbage to construct head cabbage-Chinese flowering cabbage heterograft.Based on transcriptome sequencing read library of Chinese flowering cabbage and head cabbage,coding sequences and 3’UTR sequences of BcSVP and BoSVP gene were obtained by read joining.According to interspecific differential sequences of BcSVP in 3’UTR(CF and CR),The two and one transported BcSVP reads of Chinese flowering cabbage were identified from transcriptome sequencing read libraries T01 and T02 of shoot apexes of head cabbage scions of head cabbage-Chinese flowering cabbage heterograft,respectively.The endogenous transcriptional expression of BoSVP was analyzed based on interspecific differential sequences of BoSVP(GF and GR),indicating the transcriptional expression of BoSVP did not show obvious difference between homograft and heterograft.The study indicated mRNA long-transport of BcSVP in heterograft.It lays the foundation for further study of molecule mechanism and biological function of BcSVP mRNA transport in head cabbage-Chinese flowering cabbage heterograft.

关 键 词：结球甘蓝 菜心 嫁接 BcSVP mRNA运输 

分 类 号：S635.1[农业科学—蔬菜学]