猪链球菌2型强弱毒株对BALB/c小鼠肠道菌群影响的差异  

Different effects of the virulent and avirulent strains of Streptococcus suis type 2 on the intestinal microbiota in BALB/c mice

作　　者：许格 王小敏 王俣棋 徐鹏 XU Ge;WANG Xiao-min;WANG Yu-qi;XU Peng(School of Preclinical Medicine of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学,遵义563000

出　　处：《中国人兽共患病学报》2021年第7期586-592,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金地区科学基金项目(No.31860706);贵州省教育厅青年科技人才成长项目(No.黔教合KY字[2018]222);国家级大学生创新创业训练计划(No.202010661004)。

摘　　要：目的旨在探究猪链球菌2型强弱毒株对BALB/c小鼠肠道菌群的影响差异。方法本研究随机将3周龄的雌性BALB/c小鼠分为3组,即空白对照组、猪链球菌2型强毒株SS2-1感染组和弱毒株HA0609感染组。采用Illumina Miseq测序技术,测定了小鼠结肠粪便样品中微生物16S rRNA V3-4区序列,并对其微生物群落结构和多样性进行了比较分析。结果各组样本测序覆盖率均达到99%以上,实验数据可靠。三组小鼠在菌群丰度和构成上均存在差异,SS2-1感染组小鼠的肠道菌群OTU数量与Alpha多样性均高于HA0609感染组和Mock组,HA0609感染组与Mock组相似度较为接近。相对于HA0609感染组和Mock组,SS2-1感染组菌群结构改变,益生菌所占比例减少,致病菌或潜在致病菌增多,存在肠道菌群失调现象。根据LEfSe分析结果,预测变形杆菌门下的Alphaproteobacteria可作为猪链球菌2型感染的潜在鉴别生物标志。结论通过本研究,不仅对猪链球菌2型强弱毒株感染后小鼠的肠道菌群的丰度、多样性和构成有了一定的认识,而且筛选到了猪链球菌2型感染BALB/c小鼠后的潜在标志物Alphaproteobacteria,为通过调整肠道微生物治疗猪链球菌2型和预防猪链球菌2型感染提供了依据。The objective of this study was to investigate the effects of two Streptococcus suis type 2 strains,the virulent strain SS2-1 and the avirulent strain HA0609,on the intestinal microbiota in BALB/c mice.Three week old female mice were randomly divided into three groups:a control group(Mock),an SS2-1 strain infected group and an HA0609 strain infected group.The 16S rRNA v3-4 regions of the intestinal microbiota were sequenced on the Illumina Miseq platform,and the community structure and diversity of intestinal microbiota were analyzed according to the sequence data.The sequencing coverage of all samples in each group exceeded 99%,thus indicating that the experimental data were reliable.Marked differences were observed in the abundance and communities of intestinal microbiota among the three groups.Both the number of OTU and the alpha diversity of the intestinal microbiota were higher in the SS2-1 group than the control and HA0609 groups.The HA0609 group showed greater similarity to the control group.An increased proportion of pathogenic bacteria or potential pathogenic bacteria and a decreased proportion of probiotics were detected in the SS2-1 group,thus demonstrating the imbalance in the intestinal microbiota.According to the results of LEfSe analysis,Alphaproteobacteria may be used as a potential biomarker for diagnosis of Streptococcus suis type 2 infection.The results of this study not only provide insights into the abundance,diversity and composition of the intestinal microbiota of BALB/c mice infected with Streptococcus suis type 2 virulent or avirulent strains,but also identify the potential biomarker Alphaproteobacteria for diagnosis of Streptococcus suis type 2.This study provides experimental data for improving treatment and preventing Streptococcus suis type 2 infection by adjusting microorganisms.

关 键 词：猪链球菌2型 16S rRNA 肠道菌群 BALB/C小鼠 

分 类 号：S852[农业科学—基础兽医学]