云南省部分猪场病料猪圆环病毒3型PCR检测及克隆测序  

Detection,Cloning and Sequencing of PCV3 in Some Swine Farms in Yunnan Province

作　　者：陶杰 张敬寒 彭俊文 刘艳丽 李枝兰 吕念词 张以芳 邹丰才 柴俊 Tao Jie;Zhang Jinghan;Peng Junwen;Liu Yanli;Li Zhilan;Lu Nianci;Zhang Yifang;Zou Fengcai;Chai Jun(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Mojiang Animal Disease Prevention and Control Center,Mojiang,Yunnan 654800,China;Mojiang Animal Husbandry Workstation Mojiang,Yunnan 654800,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]墨江县动物疫病预防控制中心,云南墨江654800 [3]墨江县畜牧工作站,云南墨江654800

出　　处：《中国动物检疫》2021年第8期4-7,共4页China Animal Health Inspection

基　　金：国家重点研发计划专项(2017YFD0500105);云南省专家工作站项目(202005AF150041)。

摘　　要：为了解猪圆环病毒3型(porcine circovirus type 3,PCV3)在云南省的流行及遗传进化情况,2020年10—12月收集云南省部分猪场疑似病料共24份,采用PCR方法进行PCV3病原检测;选取PCV3阳性扩增产物进行克隆测序,并将测序结果与16株国内外参考毒株进行ORF2核苷酸序列同源性分析。结果显示:24份病料样品中检出阳性5份,阳性检出率为20.8%;克隆出的阳性扩增产物(YNJS-2020)ORF2核苷酸序列和国内外参考毒株的同源性较高,为97.4%~98.9%,其中与西班牙毒株MF80572000同源性最高,与安徽14-201611毒株同源性最低。结果表明,云南省存在PCV3流行,且流行毒株与国内外其他毒株遗传关系较近,但也有一定差异。结果提示,云南省应加强对PCV3的监测及其流行的控制,避免PCV3大面积流行。本研究为云南省PCV3基因型分析和疫苗研究提供了一定的数据基础。In order to identify the status of prevalence and genetic evolution of porcine circovirus type 3 PCV3 in Yunnan Province 24 suspected samples were collected from some swine farms during October to December 2020 to detect PCV3 by PCR.PCV3-positive amplified products were cloned and sequenced and the results were compared with 16 domestic and foreign reference strains for ORF2 nucleotide sequence homology analysis.The results showed that 5 out of 24 samples were positive,with a positive detection rate of 20.8%.The ORF2 nucleotide sequence of cloned positive amplified product(YNJS-2020)had high homology with the reference strains(97.4%to 98.9%),and the homology was highest with Spanish PCV3 strain MF80572000 and lowest with Anhui strain 14-201611.It was concluded that PCV3 was prevalent in the province which had a close genetic relationship with other viral strains although there was a certain gap.It was suggested that PCV3 should be intensively monitored and controlled to avoid any spreading on a large scale.A certain data were hereby provided for PCV3 genotype analysis and development of corresponding vaccines in Yunnan Province.

关 键 词：圆环病毒3型 PCR 克隆 同源性分析 

分 类 号：S852.65[农业科学—基础兽医学]