Simultaneous determination of six steroidal saponins and one ecdysone in Asparagus filicinus using high performance liquid chromatography coupled with evaporative light scattering detection  

作　　者：Libo Zhou Zhihong Cheng Daofeng Chen 

机构地区：[1]Department of Pharmacognosy,School of Pharmacy,Fudan University,Shanghai 201203,China

出　　处：《Acta Pharmaceutica Sinica B》2012年第3期267-273,共7页药学学报（英文版）

基　　金：This investigation was supported by the grants from the Science and Technology Commission of Shanghai Municipality(04DZ19806);the Natural Science Foundation of China(30925042);the State Key Program from the Ministry of Science and Technology,China(2009ZX09502-013).

摘　　要：A high-performance liquid chromatography coupled with an evaporative light scattering detector(HPLC-ELSD)has been developed to evaluate the quality of Asparagus filicinus through a simultaneous determination of six steroidal saponins and one ecdysone,including aspafiliosides A,B,C,E,G,filiasparoside A and 20-hydroxyecdysone.With a C_(18) analytical column,the seven analytes were separated efficiently using acetonitrile-water as the mobile phase in a gradient program.The method limits of detection ranged 0.1250.225μg,and the method limits of quantitation ranged 0.408-0.720μg,respectively.The intra-and inter-day precisions of the method were evaluated and were all less than 3%.All the recoveries for the spiked analytes ranged 95.16%-100.61%.The proposed method was succesfully applied to quantify the seven components in thirteen samples from different localities in China.

关 键 词：HPLC-ELSD Asparagus filicinus Steroidal saponin ECDYSONE Aspafiliosides Filiasparoside A 20-HYDROXYECDYSONE Quality evaluation 

分 类 号：O62[理学—有机化学]