Chemical comparison of dried rehmannia root and prepared rehmannia root by UPLC-TOF MS and HPLC-ELSD with multivariate statistical analysis  

作　　者：Qiande Liang Jing Ma Zengchun Ma Yuguang Wang Hongling Tan Chengrong Xiao Ming Liu Beibei Lu Boli Zhang Yue Gao 

机构地区：[1]Institute of Radiation Medicine,Academy of Military Medical Science,Beijing 100850,China [2]Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China

出　　处：《Acta Pharmaceutica Sinica B》2013年第1期55-64,共10页药学学报（英文版）

基　　金：The authors are grateful for financial support from the National Nature Science Foundation of China(Grant Nos.81073161,81130067 and 30730112);the National Basic Research Program of China(Grant No.2011CB505304);the Natural Science Foundation of Beijing(Grant No.7112110);for technical support from Mr.Yong Wang and other technologists of Waters China Ltd.

摘　　要：To identify the chemical differences which lead to the different therapeutic effects of dried rehmannia root(DRR)and prepared rehmannia root(PRR),we compared the chemical composition of decoctions of randomly purchased DRR and PRR using ultra performance liquid chromatography(UPLC)coupled with time-of-fight mass spectrometry and high performance liquid chromatography(HPLC)coupled with evaporative light scattering detection(ELSD)with the aid of multivariate statistical analysis.Both approaches clearly revealed compositional and quantitative differences between DRR and PRR.UPLC-MS data indicated stachyose,rehmaiono-side A(or rehmaionoside B),acteoside(or forsythiaside,or isoacteoside),6-O-coumaroylajugol(or 6-O-E-feruloylajugol,or 6-O-Z-feruloylajugol)as important discriminators between DRR and PRR decoctions.HPLC-ELSD analysis showed that the content of fructose in the decoctions of PRR was about four times greater than that of DRR(P<10^(-5)),while sucrose content in the decoctions of PRR was only about one seventh of that in DRR(P<0.01).Our results suggest that some compounds,such as fructose,stachyose and rehmaionoside,may be responsible for the differing therapeutic effects of DRR and PRR.Furthermore,improvements in quality control for PRR,which is currently lacking in the Chinese Pharmacopoeia,are recommended.

关 键 词：Rehmannia root Liquid chromatography Mass spectrometry Evaporated light scattering detection Multivariate statistical analysis 

分 类 号：R28[医药卫生—中药学]