养血解毒汤对小鼠银屑病样皮损IL-36表达的影响  

Effect of YangxueJiedu Decoction on the expression levels of IL-36 in psoriasis-like lesions in mice

作　　者：张琪 朱磊 黄钰淇 陈永锋 ZHANG Qi;ZHU Lei;HUANG Yuqi;CHEN Yongfeng(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China;Guangdong Medical University,Zhanjiang 524023,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091 [2]广东医科大学,广东湛江524023

出　　处：《皮肤性病诊疗学杂志》2021年第3期164-169,共6页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省中医药局科研项目(20181181)。

摘　　要：目的:探讨养血解毒汤对小鼠银屑病样模型皮损IL-36表达的影响。方法:将18只小鼠随机分为对照组、模型组及实验组,每组6只,对各组小鼠皮损进行PASI评分;通过RT-qPCR比较各组小鼠皮损中IL-36α、IL-36β、IL-36γ与IL-36Ra mRNA表达差异;通过Western blot及免疫组化比较各组小鼠脱毛区域皮肤IL-36β的蛋白表达。结果:实验组小鼠皮损PASI评分(1.17±0.37)明显低于模型组(7.83±0.69),差异有统计学意义(t=19.07,P<0.05)。实验组IL-36β的mRNA表达(1.85±0.28)较模型组(4.85±0.42)明显降低(t=8.01,P<0.01),而IL-36α、IL-36γ及IL-36Ra的mRNA水平无明显改变。IL-36β在模型组中大量表达于颗粒层以及棘层中上部的细胞胞核和胞浆,且真皮层也有少量分布;在实验组中少量表达于真皮层,少量或无表达于表皮层。与模型组(3.27±0.32)相比,实验组小鼠皮损中IL-36β蛋白表达(2.50±0.24)明显降低(t=3.31,P<0.05)。结论:养血解毒汤通过下调IL-36β表达水平,有效改善小鼠银屑病样皮损严重程度,降低皮损PASI评分。Objective:To explore the effect of YangxueJiedu decoction on IL-36 expression in a mouse model of imiquimod-induced psoriasis-like lesions.Methods:Eighteen mice were randomly divided into the control,model and experimental groups,with 6 mice per group.The psoriasis severity was assessed by PASI score.The expression levels of mRNA for IL-36α,IL-36β,IL-36γ,and IL-36 Ra were measured by RT-qPCR.Expression levels of IL-36βprotein were evaluated by both Western blot and immunohistochemistry.Results:PASI scores were significantly lower in experimental group than in the model group(1.17±0.37 vs 7.83±0.69,t=19.07,P<0.05).Likewise,the expression levels of IL-36βmRNA were also significantly lower in the experimental group than in the model group(1.85±0.28 vs 4.85±0.42,t=8.01,P<0.01).However,expression levels of IL-36α,IL-36γand IL-36 Ra mRNA were comparable between the experimental group and the model group.In the model group,IL-36βwas mainly expressed in the nucleus and cytoplasm of the granular layer and the middle and upper spinous layers,with little expression in the dermis.In the experimental group,the dermis expressed little IL-36β,while the epidermis expressed very little or was absence of IL-36β.Consistent with immunohistochemistry,expression levels of IL-36βwere significantly lower in the experimental group than in the model group(2.50±0.24 vs 3.27±0.32,t=3.31,P<0.05).Conclusion:YangxueJiedu decoction can decrease PASI scores by down-regulation of IL-36βexpression in imiquimod-induced psoriasis-like lesions.

关 键 词：银屑病 小鼠 养血解毒汤 IL-36 

分 类 号：R285.5[医药卫生—中药学]