毛细管胶束电动色谱法同时分离检测补骨脂及其制剂中的5种活性成分  

Simultaneous separation of five active ingredients in Psoralea Fructus and its preparations by micellar capillary electrophoresis

作　　者：易婷 曹秋娥[1] 李菲[1] YI Ting;CAO Qiu-e;LI Fei(School of Chemical Science and Technology,Yunnan University,Kunming 650500,Yunnan,China)

机构地区：[1]云南大学化学科学与工程学院,云南昆明650500

出　　处：《云南大学学报（自然科学版）》2021年第4期781-787,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省教育厅科学研究基金(2017ZZX221).

摘　　要：建立了一个同时分离检测补骨脂素、补骨脂甲素、补骨脂乙素、补骨脂定和补骨脂宁5种活性成分的毛细管胶束电动色谱方法.方法以20 mmol/L Na_(2)B_(4)O_(7)(用NaOH调节pH 9.31)为缓冲溶液,加入体积比为25%的乙腈和25 mmol/L SDS为添加剂,采用20 kV分离电压,25℃的毛细管柱温,5 s压力进样(3.448 kPa),300 nm的检测波长,在23 min内实现了5种活性成分的同时分离检测,各组分峰面积与质量浓度呈良好的线性关系.同时将方法应用于补骨脂药材及其复方制剂中这5种活性成分的分析,各组分加标回收试验回收率为95.2%~104.8%,相对标准偏差小于5%,说明所建立的新方法准确、可靠,可用于补骨脂药材及其制剂中这5种活性成分的质量控制.A new micellar capillary electrophoresis method was developed for simultaneous separation and determination of psoralin,coryfolin,corylifolinin,psoralidin and corylin in Psoraleae Fructus within 23 minutes by using a Na_(2)B_(4)O_(7)(20 mmol/L)buffer(adjust pH to 9.31 with NaOH)consisting of 25%(v/v)acetonitrile,25 mmol/L SDS in the instrumental conditions of the separation voltage of 20 kV,detector wavelength of 300 nm,and injection of 5 s at 3.448 kPa.The developed method was applied to the determination of five compounds in Psoraleae Fructus and its preparations with the recovery in the ranged between 95.4%−104.7% and the relative standard deviation of less than 5.0%.So this new method is accurate and reliable,and can be used for the quality control of Psoraleae Fructus and its preparations.

关 键 词：毛细管胶束电动色谱 补骨脂 复方制剂 

分 类 号：O657.7[理学—分析化学]