反硝化微生物菌剂提升A^(2)/O工艺TN去除效果  

Denitrifying microbial agents improving TN removal of A^(2)/O process

作　　者：车林 金文标 陈洪一 迟惠中 梁云跃 CHE Lin;JIN Wenbiao;CHEN Hongyi;CHI Huizhong;LIANG Yunyue(Shenzhen Engineering Laboratory of Microalgal Bioenergy,Harbin Institute of Technology(Shenzhen),Shenzhen 518055,Guangdong,China)

机构地区：[1]哈尔滨工业大学(深圳)深圳微藻生物能源工程实验室,广东深圳518055

出　　处：《化工学报》2021年第S01期467-474,共8页CIESC Journal

基　　金：深圳市科技创新委员会治水提质科技专项(KJYY20171011144235970)。

摘　　要：反硝化是污水脱氮的关键步骤。投加反硝化微生物菌剂是提升总氮去除效率的有效方法。本实验从城市污水处理厂A^(2)/O工艺厌氧池和缺氧池获取活性污泥并筛选出Pseudomonas aeruginosa F5(以下简称F5)。构建以实际生活污水为基础的培养基培养F5,通过正交试验的方式确定关键营养物质的最适投加量。研究发现,牛肉膏、K_(2)SO_(4)和MgSO_(4)·7H2O的最适投加量分别为5.0 g/L、6.7 mg/L和5.0 mg/L。为验证F5作为反硝化微生物菌剂的可行性,在50 m^(3)A^(2)/O中试反应器中以1∶10000(V菌剂/V日处理污水)比例连续投加F5,总氮去除率提升了19.6%~24.0%。为进一步提高反硝化微生物菌剂的效能,采用常压室温等离子体育种法诱变F5后获得两株诱变菌株A75和A82。结果表明,A75和A82的总氮去除率较F5分别提高了14.5%~16.2%和16.8%~18.0%。该研究为污水处理厂提升氮排放标准提供了可靠的理论依据。Denitrification is a key step in sewage denitrification.Adding denitrifying microbial agents is an effective method to improve the removal efficiency of total nitrogen.In this experiment,Pseudomonas aeruginosa F5(F5 in short hereafter)was harvested from the anaerobic/anoxic tank of an A^(2)/O process in a municipal sewage treatment plant.A culture medium based on actual domestic sewage was prepared to cultivate F5,and the optimal dosage of key nutrients were determined through orthogonal experiments.The optimum dosage of beef extract,K_(2)SO_(4) and MgSO_(4)·7H_(2)O were 5.0 g/L,6.7 mg/L and 5.0 mg/L,respectively.In order to verify the feasibility of denitrification,F5 was added continuously to a pilot-scale(50 m^(3))A^(2)/O reactor at a ratio of 1∶10000(Vinoculant/Vdaily sewage).The addition of F5 increased the total nitrogen removal rate by 19.6%-24.0%.For further improving denitrification efficiency,two mutant strains A75 and A82 were obtained after mutagenizing F5 by atmospheric and room temperature plasma method(ARTP).The results showed that the total nitrogen removal rate increased by 14.5%-16.2%and 16.8%-18.0%,respectively,by adding A75 and A82 compared with F5.This research provides a reliable and theoretical basis for sewage treatment plants to improve denitrification capability.

关 键 词：反硝化细菌 总氮 A^(2)/O 常压室温等离子体育种法 微生物菌剂 

分 类 号：X703.1[环境科学与工程—环境工程]