黄芩苷介导Nrf2/HO-1通路的激活增强丙泊酚对肾缺血再灌注损伤大鼠的保护作用  

Propofol′s Protective Effect Enhancement by Baicalin Mediated Activation of Nrf2/HO-1 Pathway on Renal Ischemia-reperfusion Injury Rats

作　　者：李前辉[1] 张宜林[1] 谢小娟[1] 郭浩翔 王翔宇 LI Qianhui;ZHANG Yilin;XIE Xiaojuan;GUO Haoxiang;WANG Xiangyu(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang Henan China 471023)

机构地区：[1]河南科技大学第一附属医院,河南洛阳471023

出　　处：《中医学报》2021年第8期1704-1710,共7页Acta Chinese Medicine

基　　金：河南省教育厅自然科学研究计划项目(2015B20235)。

摘　　要：目的:探究黄芩苷(baicalin,BAI)联合丙泊酚(propofol,Prof)对肾缺血再灌注损伤(renal ischemia-reperfusion injury,RIRI)大鼠的影响。方法:将大鼠随机分为5组:Sham组、BAI(30 mg·kg^(-1))组、RIRI组、RIRI+Prof(50 mg·kg^(-1))组和RIRI+Prof+BAI(Prof,50 mg·kg^(-1)+BAI,30 mg·kg^(-1))组,除Sham组和BAI组外,其余大鼠建立肾缺血再灌注损伤模型,连续给予相应的药物7 d。检测大鼠尿液中尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)水平;血清中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)水平;TUNEL染色检测大鼠肾组织细胞凋亡;Western Blot法检测大鼠肾组织Bax、Bcl-2、p-Nrf2、HO-1、NQO1蛋白表达。加入Nrf2/HO-1通路抑制剂ML385,检测尿液中BUN、Scr水平,血清中MDA、SOD含量;HE染色观察肾组织病理损伤;Western Blot法检测大鼠肾组织Nrf2、p-Nrf2、HO-1、NQO1蛋白表达;TUNEL染色检测肾组织细胞凋亡。结果:BAI联合Prof可改善RIRI大鼠肾组织病理损伤程度,降低尿液BUN、Scr水平及血清中MDA含量、肾组织细胞凋亡率,升高血清中SOD活力及肾组织HO-1、NQO1蛋白表达、p-Nrf2/Nrf2、Bcl-2/Bax水平;加入Nrf2/HO-1通路抑制剂则能逆转BAI联合Prof对RIRI大鼠的保护作用。结论:BAI介导Nrf2/HO-1通路的激活增强Prof对RIRI大鼠的保护作用。Objective:To explore the effects of baicalin(BAI)combined with propofol(Prof)on renal ischemia-reperfusion injury(RIRI)rats.Objective:To investigate the effects of Baicalin combined with propofol on renal ischemia-reperfusion injury(RIRI)in rats.Methods:Methods:The rats were randomly divided into 5 groups:Sham group,BAI(30 mg·kg^(-1))group,RIRI group,RIRI+Prof(50 mg·kg^(-1))group,and RIRI+Prof+BAI(Prof,50 mg·kg^(-1)+BAI,30 mg·kg^(-1))group.Except for the Sham group and the BAI group,the rest other rats have established a RIRI model,and the corresponding drugs were continuously given for 7 days.Blood urea nitrogen(BUN),serum creatinine(Scr)levels in rat urine,and serum superoxide dismutase(SOD)and malondialdehyde(MDA)levels were detected.TUNEL staining was used to detect cell apoptosis in rat kidney tissue.Western Blot method was used to detect the protein expression of Bax,Bcl-2,p-Nrf2,HO-1 and NQO1 in rat kidney tissue.The Nrf2/HO-1 pathway inhibitor ML385 was added to detect the protein expression of Nrf2,p-Nrf2,HO-1 and NQO1 in rat kidney tissue;TUNEL staining was used to detect renal cell apoptosis.The BUN and Scr levels in urine and MDA and SOD content in serum were detected.And HE staining was used to observe pathological damage of kidney tissue.Results:BAI combined with Prof can alleviate the pathological damage degree of renal tissue in RIRI rats,reduce urine BUN and Scr levels,serum MDA content,renal cell apoptosis rate,increase the serum SOD activity and renal tissue HO-1,NQO1 protein Expression,p-Nrf2/Nrf2,Bcl-2/Bax levels,however,adding Nrf2/HO-1 pathway inhibitor ML385 can reverse the protective effect of BAI combined with Prof on RIRI rats.Conclusions:Baicalin-mediated activation of the Nrf2/HO-1 pathway enhances the protective effect of propofol on RIRI in rats.

关 键 词：黄芩苷 丙泊酚 肾缺血再灌注 Nrf2/HO-1通路 

分 类 号：R285.5[医药卫生—中药学]