New Cassane Diterpenoids from the Seed Kernels of Caesalpinia cucullata, Exhibit Anti-inflammatory Effect in vitro by Inhibiting iNOS Enzymatic Activity  

作　　者：Miao Wang Yi-Ren Yang Yan Yin Kai-Ru Song Li-Ping Long Xian-Zhe Li Bei Zhou Hui-Yuan Gao 

机构地区：[1]School of Traditional Chinese Moterio Medico,Shenyang Pharmaceutical University,Shenyang,Liaoning 110016,China [2]Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of Education,Shenyang Pharmoceuticol University,Shenyang,Liaoning 110016,China

出　　处：《Chinese Journal of Chemistry》2021年第6期1625-1634,共10页中国化学（英文版）

基　　金：the National Natural Science Foundation of China(No.31670359);Liao Ning Revitalization Talents Program(No.XLYC 1905019);Natural Science Foundation of Liaoning Province(No.201602691);The authors wish to thank Dr.Jiuzhi Yuan(Shenyang Pharmaceutical University,Shenyang,China)for species identification of the plant materials.

摘　　要：Inflammation is a complex biological response to stimulation.Natural cassane diterpenoids from Caesalpinia genus exhibit significant anti-inflammation activity.Eight new cassane diterpenoids(1-8)along with seven known ones(9-15)were obtained from the seed kernels of Caesalpinia cucullata Roxb.This is the first report on chemical investigation of the seed kernels of C.cucullata,and the cassane diterpenes were found in this plant for the first time.Their structures were elucidated based on the extensive spectroscopic analyses,and the absolute configurations were identified by ECD calculation and X-ray crystallography.All compounds were evaluated for their anti-inflammation activity by inhibiting NO production in LPS-induced RAW 264.7 cells.Compounds 1-2 and 9-11 exhibited effective inhibitory activity with inhibition rate more than 50%.The iNOS enzyme activity and molecular docking experiments were performed to explore the preliminary mechanism.Eventually,a potential anti-inflammatory mechanism revealed that the bioactive cassane inhibited overproduction of NO by targeting key residues in the iNOS active cavity to reduce iNOS enzymatic activation.

关 键 词：Caesalpinia cucullata Roxb. Cassane diterpenoids Structure elucidation Biological activity iNOS enzyme activity 

分 类 号：O62[理学—有机化学]