Antimicrobial Chlorinated Carbazole Alkaloids from the Sponge-Associated Actinomycete Streptomyces diacarni LHW51701  

作　　者：Yijia Cheng Nannan Chen Jing Li Jun-Cheng Su Jingya Yang Cui-Xian Zhang Hou-Wen Lin Yongjun Zhou 

机构地区：[1]School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006,China [2]Research Center for Marine Drugs,State Key Laboratory of Oncogenes and Related Genes,Depanment of Pharmacy,Ren Ji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China [3]College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Chinese Journal of Chemistry》2021年第5期1188-1192,共5页中国化学（英文版）

基　　金：the National Key R&D Program of China(No.2019YFC0312501);the National Natural Science Foundation of China(No.31929001);the Special Project for Marine Economic Development(Six Major Marine Industries)of Guangdong Province Department of Natural Resources of Guangdong Province(No.[2020]039);the Construction Project of Shanghai Key Laboratory of Molecular Imaging(No.18DZ2260400);the Innovative Research Team of High-Level Local Universities in Shanghai,and the Taishan Scholar Project from Shandong Province to H.L.The authors are grateful to Ying Wu for QTOF mass analysis,Hong-Yan Liu for NMR analysis,and Prof.Meifeng Tao from School of Life Sciences and Biotechnology,Shanghai Jiao Tong University for kindly providing the indicator strains for bioactivity assay.

摘　　要：Four new chlorinated carbazole alkaloids,chlocarbazomycins(CCBs)A-D,were isolated from sponge associated bacterium Streptomyces diacarni LHW51701.Their structures were elucidated via spectroscopic techniques(including HRESIMS,ID&2D NMR),and X-ray crystallographic analysis.Structurally,the 4-chloro coupled with 3-methoxyl substituents in the tricycle nucleus of CCBs A-D and the A/-methoxyl group of CCB-D are rare in naturally occurred tricyclic carbazole alkaloids.Moreover,CCBs A-D do not bear the typical Cl poro-alkyl and C2 meto-methyl side chains of bacterial tricycle carbazoles,suggesting a novel mechanism of aromatic ring formation in biosynthesis.The biological evaluation showed that CCB-C possessed inhibitory activities against pathogenic microorganisms including methicillin-resistant Staphylococcus aureus(MRSA),Mycobacterium smegmatis,Bacillus mycoides,and Candida albicans.

关 键 词：ANTIBIOTICS Natural products HALOGENATION SPONGE Symbiotic microorganism 

分 类 号：O62[理学—有机化学]