基于HPLC指纹图谱与多成分含量测定结合化学计量学的玉屏风散质量评价  

Quality evaluation of Yupingfeng Powder based on HPLC fingerprint,determination of multi-index components and chemometrics analysis

作　　者：申亚君 安琪 丁笑颖 李恒阳 郭龙 郑玉光[1,2] 郑倩 张丹[1] SHEN Ya-jun;AN Qi;DING Xiao-ying;LI Heng-yang;GUO Long;ZHENG Yu-guang;ZHENG Qian;ZHANG Dan(Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Chemical and Pharmaceutical College,Shijiazhuang 050026,China;Chinese Traditional Medicine Hospital of Shijiazhuang,Shijiazhuang 050051,China)

机构地区：[1]河北中医学院河北省中药炮制技术创新中心,石家庄050200 [2]河北医药化工职业技术学院,石家庄050026 [3]石家庄市中医院,石家庄050051

出　　处：《天然产物研究与开发》2021年第7期1121-1128,共8页Natural Product Research and Development

基　　金：河北省自然科学基金青年项目(H2018423029);中央本级重大增减支项目(2060302);中央财政公共卫生专项“中药资源普查项目”(Z135080000022);河北省二期现代农业产业技术体系创新团队项目(HBCT2018060205)。

摘　　要：建立玉屏风散HPLC指纹图谱结合化学计量学的方法对其进行分析,并测定其中11种成分的含量,为玉屏风散质量控制提供科学依据。采用Shimadzu Inertsil ODS-2 C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水为流动性进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长230 nm。15批玉屏风散的HPLC指纹图谱,相似度>0.83,标定了24个共有峰,指认11个色谱峰,分别为升麻素苷、毛蕊异黄酮葡萄糖苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷、毛蕊异黄酮、芒柄花素、芒柄花苷、白术内酯III、白术内酯I、白术内酯II,含量测定结果分别为0.104~0.449、0.048~0.121、0.047~0.111、0.129~0.550、0.017~0.037、0.025~0.053、0.030~0.118、0.009~0.025、0.148~0.615、0.088~0.382、0.073~0.385。借助SPSS 23.0、SIMCA-P 13.0等软件对15批玉屏风散进行聚类分析(HCA)、主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)等化学计量学分析,15批样品聚为2类。所建立的玉屏风散质量评价方法稳定性、重复性好,可用于玉屏风散的质量控制和评价。To establish an analysis method of Yupingfeng Powder based on HPLC fingerprint combined with chemometrics analysis,and determined the contents of 11 components in it,which provides the scientific basis for quality control of Yupingfeng Powder.The chromatographic column was Shimadzu Inertsil ODS-2 C18(250 mm×4.6 mm,5μm),the mobile phase was composed of the acetonitrile-water solution in gradient elution manner at a flow rate of 1.0 mL/min,the column temperature was set at 30℃,the detection wavelength was set at 230 nm.The HPLC fingerprint of 15 batches of Yupingfeng Powder was established,the similarity was more than 0.83,24 common peaks were calibrated and 11 chromatographic peaks were identified.They are prim-O-glucosylcimifugin,calycosin-7-O-β-D-glucoside,cimifugin,5-O-methylvisammioside,sec-O-glucosylhamaudol,calycosin,formononetin,ononin,astragaloside III,astragaloside I,astragaloside II,respectively.The results of content determination were 0.104-0.449,0.048-0.121,0.047-0.111,0.129-0.550,0.017-0.037,0.025-0.053,0.030-0.118,0.009-0.025,0.148-0.615,0.088-0.382,0.073-0.385.The results were analyzed by using SPSS 23.0,SIMCA-P 13.0,and other software to perform HCA,PCA,and OPLS-DA,and other chemometric analyses of 15 batches of Yupingfeng Powder,fifteen batches of samples were grouped into two groups.The established quality evaluation method of Yupingfeng Powder is stable and reproducible and can be used for the quality control and evaluation of Yupingfeng Powder.

关 键 词：玉屏风散 HPLC 指纹图谱 含量测定 化学计量学 

分 类 号：R917[医药卫生—药物分析学]