Identification of a male-specific region(MSR)in Spinacia oleracea  

作　　者：Hongbing She Zhaosheng Xu Helong Zhang Guoliang Li Jian Wu Xiaowu Wang Ying Li Zhiyuan Liu Wei Qian 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Horticultural Plant Journal》2021年第4期341-346,共6页园艺学报（英文版）

基　　金：This work was performed at the Key Laboratory of Biol-ogy and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing,China,and was supported by the Nat-ural Science Foundation of China(Grant No.31872102);the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAAS-ASTIP-IVFCAAS);Beijing Scientific Program of Municipal Commission of Science and Technology(Grant No.Z171100001517014);Central Public-interest Scientific Institution Basal Research Fund(Grant No.IVF-BRF2018004);the National Key Research and Development Program of China(Grant No.2018YFD0100805).

摘　　要：Dioecy,the presence of male and female individual,has evolved independently from hermaphroditism in multiple flowering plant lineages.Spinach,an important leafy vegetable crop worldwide,is a dioecious species with an XY sex-determination system.Although some markers that are fully linked to male-determination locus were identified,the male-specific region(MSR)remained unclear.In this research,five male individuals and five female lines were resequenced to identify the male-specific region.We identified a region(∼21 kb)on chromosome 4(putative sex chromosome)where the five females had a low reads coverage,while the five males had high coverage.A KASP marker,SponR,developed from a single nucleotide polymorphism(SNP)closely linked to the MSR was co-segregating with the sex determination gene in the population of 958 individuals,suggesting that the MSR might be specifically present in male spinach plants.

关 键 词：Spinacia oleracea DIOECY MSR(male-specific region) Sex chromosome 

分 类 号：S636.1[农业科学—蔬菜学]