丙烯酰乙二胺-β-环糊精毛细管电色谱整体柱的制备及应用  

Preparation and application of monolithic column of acryloylethylenediamine-β-cyclodextrin capillary electrochromatography

作　　者：唐艺旻 李英杰[1] 高立娣[1] 秦世丽 赵冰[1] 靳凤龙 TANG Yimin;LI Yingjie;GAO Lidi;QIN Shili;ZHAO Bing;JIN Fenglong(School of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006,China;Inspection and Testing Center of Food and Drug,Qiqihar Market Superrision Administration,Qiqihar 161005,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006 [2]齐齐哈尔市场监督管理局食品药品检验检测中心,齐齐哈尔161005

出　　处：《黑龙江大学自然科学学报》2021年第3期294-299,共6页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(21978140);黑龙江省省属高等学校基本科研业务费资助项目(135409205)。

摘　　要：合成了丙烯酰乙二胺-β-环糊精(6-EA-β-CD),通过红外光谱和质谱对其结构进行表征。采用一步键合法制备了6-EA-β-CD毛细管电色谱整体柱,优化制柱条件。采用扫描电镜对整体柱柱内固定相形貌进行表征。以硫脲、D,L-丙氨酸和盐酸班布特罗对映体对柱性能进行评价。结果表明,该制备方法简单,且所得的6-EA-β-CD毛细管电色谱整体柱具有较强的手性拆分能力。Acrylethylenediamine-β-cyclodextrin(6-EA-β-CD)was synthesized,and its structure was characterized by infrared spectroscopy and mass spectrometry.The monolithic column of 6-EA-β-CD capillary electrochromatography was prepared by one-step bonding method,and the column preparation conditions were optimized.The morphology of stationary phase in the monolithic column was characterized by scanning electron microscope.The performance of the column was evaluated with thiourea,D,L-alanine and bambuterol hydrochloride.The results showed that the preparation method was simple,and the obtained monolithic column of 6-EA-β-CD capillary electrochromatography exhibited an excellent ability of enantioseparation and simple operation.

关 键 词：丙烯酰乙二胺-β-环糊精 毛细管电色谱 整体柱 对映体拆分 

分 类 号：O657.8[理学—分析化学]